{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:03:37Z","timestamp":1784887417875,"version":"3.55.0"},"reference-count":52,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201024\u2010CE29\u20105467\u201001"],"award-info":[{"award-number":["ANR\u201024\u2010CE29\u20105467\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201023\u2010CE29\u20100027"],"award-info":[{"award-number":["ANR\u201023\u2010CE29\u20100027"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small Methods"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Lipid nanoparticles (LNPs) are the cornerstone of RNA\u2010based vaccine delivery, yet their internal structure, particularly in the presence of large, single\u2010stranded messenger RNA (mRNA), remains poorly understood. Here, we apply relayed dynamic nuclear polarization (DNP)\u2010enhanced solid\u2010state NMR to directly detect encapsulated mRNA and map the spatial distribution of lipid and nucleic acid components within intact LNPs formulated with clinically relevant excipients. By refining cryoprotectant removal and polarizing matrix composition, we report the solid\u2010state NMR observation of the mRNA cargo within functional LNPs, with clear C1\u2032\u2010H1\u2032 correlation confirming resonance assignment. Quantitative analysis of polarization enhancements and build\u2010up kinetics reveals a concentric architecture: PEG\u2010lipids reside at the surface, helper lipids (DOPE, cholesterol) in intermediate layers, and mRNA within the core. These experimental trends are reinforced by numerical simulations that model the polarization transport by spin diffusion across a library of eight structural models. A multi\u2010layered core\u2013shell configuration, placing mRNA at the center surrounded by stratified lipid shells, best fits the full set of NMR observables. Together, these results confirm relayed\u2010DNP MAS NMR as a powerful method for probing nanometer\u2010scale spatial organization in LNPs and provide a quantitative structural framework to guide the rational design of RNA delivery systems.<\/jats:p>","DOI":"10.1002\/smtd.70797","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T07:01:07Z","timestamp":1782284467000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mapping mRNA Localization and Internal Structure in Lipid Nanoparticles through Solid\u2010State Dynamic Nuclear Polarization NMR and Proton Spin\u2010Diffusion Modeling"],"prefix":"10.1002","volume":"10","author":[{"given":"Adrienn","family":"Rancz","sequence":"first","affiliation":[{"name":"CNRS, ENS Lyon CRMN (Centre de RMN \u00e0 Hauts Champs de Lyon UMR 5082) Universit\u00e9 Claude Bernard Lyon 1  Villeurbanne France"},{"name":"Laboratoire de Biophotonique et 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