{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T08:14:26Z","timestamp":1788509666036,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201017\u2010EURE\u2010002"],"award-info":[{"award-number":["ANR\u201017\u2010EURE\u2010002"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small Methods"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Precise control over fluorophore localization within polymer nanoparticles remains a major challenge, limiting the rational optimization of brightness, F\u00f6rster Resonance Energy Transfer (FRET), and biosensing performance. Here, we report architecturally controlled donor core\u2010shell\u2010crown fluorescent polymer nanoparticles synthesized via a controlled radical polymerization in miniemulsion through a one\u2010pot process performed entirely in water at high solid content. The polymerizable BODIPY monomers are covalently incorporated into the polymer backbone, preventing dye leakage while enabling spatial confinement within defined compartments. By selectively positioning the dye in the core, the shell, or both, we decouple brightness and donor\u2010acceptor distance effects. Core\u2010confined dyes maximize brightness by reducing water\u2010induced quenching, whereas shell\u2010localized dyes enhance FRET efficiency (up to 75%) by minimizing donor\u2010acceptor separation, revealing a brightness\/distance trade\u2010off analogous to that observed in inorganic nanomaterials. Exciton migration and multivalent surface functionalization further amplify energy transfer within each nano\u2010object. In a competitive biotin\u2010streptavidin proof of concept assay, nanoparticle brightness emerges as the dominant parameter controlling analytical sensitivity. The optimized sensor achieves a limit of detection of \u223c4\u20138\u00a0nM in different buffers or artificial fluids, and using both a spectrofluorometer and a low\u2010cost LED\u2010based device, highlighting the potential of these scalable, metal\u2010free nanotransducers for point\u2010of\u2010care biosensing.<\/jats:p>","DOI":"10.1002\/smtd.70865","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:52:10Z","timestamp":1785131530000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization of Fluorescent Nanoparticles Architecture for Efficient Brightness, FRET Efficiency and Biosensing"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6154-2328","authenticated-orcid":false,"given":"Dalel El","family":"Marrouki","sequence":"first","affiliation":[{"name":"CNRS Bordeaux INP ISM University of Bordeaux  Talence France"},{"name":"CNRS Bordeaux INP LCPO University of Bordeaux  Pessac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8032-3744","authenticated-orcid":false,"given":"Clara","family":"Catros","sequence":"additional","affiliation":[{"name":"CNRS Bordeaux INP ISM University of Bordeaux  Talence France"},{"name":"CNRS Bordeaux INP LCPO University of Bordeaux  Pessac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Sanchez","sequence":"additional","affiliation":[{"name":"CNRS Bordeaux INP ISM University of Bordeaux  Talence France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5796-7961","authenticated-orcid":false,"given":"Paul","family":"Marque","sequence":"additional","affiliation":[{"name":"CNRS Bordeaux INP LCPO University of Bordeaux  Pessac 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