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From encoding to storage, massive information stored in synthetic DNA requires low\u2010loss and high\u2010speed transmission to a reliable preservation environment. Minimizing energy consumption remains a key challenge throughout this process. In this study, an aqueous biphasic interfacial transmission (BIT) pathway is established that enables simultaneous salt\u2010decontaminated migration and anti\u2010aging preservation, with a theoretically extrapolated half\u2010life of up to 577 years at \u221220\u00b0C. Specific temperature windows of either 20\u00b0C, 25\u00b0C, or 30\u201340\u00b0C open the trans\u2010phasic pathway. Covering most room\u2010temperature scenarios without excess energy input, the process is completed at a 30\u2010minute standing solution state or accelerated by a 2\u2010minute centrifugation mode. The interphase partition coefficient exceeds 264, ensuring a DNA transfer efficiency of over 80% regardless of DNA structure or sequence. The low water activity of the PEG\u2010rich preservation environment stabilizes the nucleotide bonding, thus preserving the integrity of DNA's secondary structure information throughout the entire phase transferring process. The polymeric preservation network kinetically restricts DNA and enzyme diffusion, isolating degradation factors. This BIT pathway enables a scalable and energy\u2010efficient strategy with tunable operational parameters for securing DNA data storage during information circulation and long\u2010term preservation.<\/jats:p>","DOI":"10.1002\/smtd.70866","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T14:02:58Z","timestamp":1784901778000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biphasic Interfacial Transmission Enables DNA Storage in Liquid"],"prefix":"10.1002","volume":"10","author":[{"given":"Yanan","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Chemistry College of Sciences Northeastern University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Sciences Northeastern University  Shenyang 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