{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:00:31Z","timestamp":1790172031876,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small Methods"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Determination of the concentration of a biomolecule in a sample is a fundamental measurement in analytical chemistry and central to many diagnostic applications. Solid\u2010state nanopores (ssNPs) stand out as leading candidates to quantify biomolecules due to their capacity to achieve label\u2010free electrical detection with single\u2010molecule resolution. Often, the concentration of a biomolecular sample is estimated in relation to the ssNP capture rate, that is, the number of molecules translocated by the nanopore per unit time. However, this method poses challenges due to variability in capture rates, which have proven difficult to control experimentally. In this work, we introduce a microtrap, that is, a microfluidic device with an integrated nanopore sensor, that leverages sample compartmentalization in known pL volumes allowing direct molecule counting and eliminating the need for capture rate calibration. We demonstrate direct quantification of pico\u2010 to nanomolar concentrations of double\u2010stranded DNA (dsDNA) and green fluorescent protein (GFP) molecules in minutes and without the need for calibration of the nanopore sensor capture rate. Moreover, we demonstrate the ability to preconcentrate the biomolecular sample by real\u2010time tuning of the microtrap volume, which can be used to reduce the measurement time and to lower the detection limit.<\/jats:p>","DOI":"10.1002\/smtd.70924","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T05:30:36Z","timestamp":1786512636000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microfluidic Sample Compartmentalization for Biomolecular Concentration Quantification Using a Nanopore Sensor"],"prefix":"10.1002","volume":"10","author":[{"given":"Morteza","family":"Safari","sequence":"first","affiliation":[{"name":"Ottawa\u2010Carleton Institute for Biomedical Engineering  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Chawich","sequence":"additional","affiliation":[{"name":"Department of Physics University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Najafi Sohi","sequence":"additional","affiliation":[{"name":"Department of Physics University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Charron","sequence":"additional","affiliation":[{"name":"Department of Physics University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Tabard\u2010Cossa","sequence":"additional","affiliation":[{"name":"Ottawa\u2010Carleton Institute for Biomedical Engineering  Ottawa Ontario Canada"},{"name":"Department of Physics University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9360-0675","authenticated-orcid":false,"given":"Michel","family":"Godin","sequence":"additional","affiliation":[{"name":"Ottawa\u2010Carleton Institute for Biomedical Engineering  Ottawa Ontario Canada"},{"name":"Department of Physics University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112901"},{"key":"e_1_2_8_3_1","first-page":"73","volume-title":"Methods in Enzymology","author":"Noble J. 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