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In this study, we present an integrated and broadly applicable microfluidic system capable of real\u2010time electrical profiling and on\u2010demand acoustic sorting across multiple physical and functional scales, from cell type, cellular biomechanics to molecularly regulated membrane\u2010functional states. The system achieved up to 98% purity in size\u2010based sorting, and accurately distinguished normal and perturbed erythrocytes exhibiting rigidity\u2010associated impedance shifts induced by chemical crosslinking, oxidation, or ion\u2010mediated regulation. At the level of molecularly regulated membrane function, we introduced an optogenetic model and achieved label\u2010free sorting of optogenetically induced and pharmacologically modulated cell states based on protein\u2010mediated membrane electrical responses. 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