{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:54:36Z","timestamp":1791431676402,"version":"4.3.3"},"reference-count":34,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22404066"],"award-info":[{"award-number":["22404066"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32471430"],"award-info":[{"award-number":["32471430"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small Methods"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Illegal use of anabolic\u2010androgenic steroids (AASs) to feed livestock can lead to food residues and endanger public health. Thus, the development of rapid, sensitive detection methods for AASs is urgently needed for AASs to safeguard food safety. In this study, four novel haptens were designed for six AASs (androstenedione, 1,4\u2010androstadienedione, boldenone, methandienone, testosterone, and methyltestosterone). By integrating computer simulation with animal immunization, we identified an optimal hapten combination and generated a hybridoma cell line capable of broadly recognizing all six AASs. Based on cell sequencing, a recombinant antibody (rAb\u20104E2) was prepared, with half\u2010maximal inhibitory concentrations of 3.37\u201322.24\u00a0ng\/mL for the six AASs. Molecular docking revealed TRP106 and VAL99 as key residues for broad\u2010spectrum recognition. A lateral flow immunochromatographic assay strip using rAb\u20104E2 was developed for AASs detection in milk, beef, and fish, achieving visual detection limits of 2\u201310\u00a0ng\/mL, 5\u201320\u00a0\u00b5g\/kg, and 10\u2013100\u00a0\u00b5g\/kg, respectively. Validation with LC\u2010MS\/MS confirmed its reliability for real samples, offering an effective approach for rapid qualitative hormone screening.<\/jats:p>","DOI":"10.1002\/smtd.71022","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:47:16Z","timestamp":1788529636000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Broad\u2010Spectrum Recombinant Antibody Profiled by Hapten Prediction and Its Application in Immunoassay for Anabolic\u2010Androgenic Steroids"],"prefix":"10.1002","volume":"10","author":[{"given":"Jialin","family":"Hu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Resources Jiangnan University  Wuxi Jiangsu China"},{"name":"International Joint Research Laboratory for Biointerface and Biodetection School of Food Science and Technology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6133-3828","authenticated-orcid":false,"given":"Xinxin","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources Jiangnan University  Wuxi Jiangsu China"},{"name":"International Joint Research Laboratory for Biointerface and Biodetection School of Food Science and Technology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5332-7101","authenticated-orcid":false,"given":"Maozhong","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources Jiangnan University  Wuxi Jiangsu China"},{"name":"International Joint Research Laboratory for Biointerface and Biodetection School of Food Science and Technology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Contact Materials Safety State Administration for Market Regulation Jiangsu Product Quality Testing &amp; Inspection Institute  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2724-8722","authenticated-orcid":false,"given":"Hua","family":"Kuang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources Jiangnan University  Wuxi Jiangsu China"},{"name":"International Joint Research Laboratory for Biointerface and Biodetection School of Food Science and Technology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5639-7102","authenticated-orcid":false,"given":"Chuanlai","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources Jiangnan University  Wuxi Jiangsu China"},{"name":"International Joint Research Laboratory for Biointerface and Biodetection School of Food Science and Technology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1930-7814","authenticated-orcid":false,"given":"Lingling","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources Jiangnan University  Wuxi Jiangsu China"},{"name":"International Joint Research Laboratory for Biointerface and Biodetection School of Food Science and Technology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2025.116377"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2024.115996"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3677"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jp274970"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac103013h"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac301984t"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664\u2010025\u201000372\u2010z"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c14481"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac500540z"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112168"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf2012437"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/09540105.2015.1137276"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2023.102498"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2025.144723"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2025.139336"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2025.117524"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c02962"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202404569"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00018-5"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.137328"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2022.129931"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c06364"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2025.147097"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003819\u20102"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.1c00203"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2024.135812"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c06442"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664\u2010025\u201000377\u20108"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c05567"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c03838"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c03986"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c09933"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biosx.2025.100660"}],"container-title":["Small Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smtd.71022","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/smtd.71022","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smtd.71022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:36:16Z","timestamp":1791430576000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smtd.71022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":34,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/smtd.71022"],"URL":"https:\/\/doi.org\/10.1002\/smtd.71022","archive":["Portico"],"relation":{},"ISSN":["2366-9608","2366-9608"],"issn-type":[{"value":"2366-9608","type":"print"},{"value":"2366-9608","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]},"assertion":[{"value":"2026-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71022"}}