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Here, we present a generally applicable RACS platform that tackles these challenges. For adaptive dual\u2010mode operation that supports diverse spontaneous Raman signals, microfluidic optical tweezers are designed with switchable capture strategies, with a high\u2010throughput in\u2010stream mode (up to 834 events\/min) for resonance Raman signals with short exposures (&lt;0.1 s) for resonance\u2010Raman\u2010based screening, and a high\u2010precision out\u2010stream mode (up to 50 events\/min) for non\u2010resonance Raman detection with extended exposures (&gt;0.1 s). Moreover, to achieve low\u2010volume concentrated harvesting, an on\u2010chip storage and centralized harvesting mechanism delivers target cells in uniform 10\u00a0\u00b5L suspensions with &gt;97% purity. Using the in\u2010stream mode for resonance Raman screening, we isolated a\n                    <jats:italic>Yarrowia lipolytica<\/jats:italic>\n                    mutant with 77% increased \u03b2\u2010carotene production in just one sorting round directly from a mutant library. Employing the out\u2010stream mode for non\u2010resonance Raman detection, we enriched phosphate\u2010solubilizing bacteria from wastewater with nearly 100% recovery in concentrated 10\u00a0\u00b5L volumes, enabling direct function\u2010targeted mini\u2010metagenomics. This high\u2010throughput dual\u2010mode RACS platform with low\u2010volume cell harvesting greatly expands the application of label\u2010free live\u2010cell sorting via metabolic phenome.\n                  <\/jats:p>","DOI":"10.1002\/smtd.71037","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T10:50:06Z","timestamp":1789210206000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High\u2010Throughput Dual Resonance and Non\u2010Resonance Mode Raman\u2010Activated Cell Sorting With Low\u2010Volume Harvesting"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3682-5309","authenticated-orcid":false,"given":"Teng","family":"Xu","sequence":"first","affiliation":[{"name":"Single\u2010Cell Center State Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences  Qingdao Shandong China"},{"name":"Shandong Energy Institute  Qingdao Shandong China"},{"name":"Qingdao New Energy Shandong Laboratory  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Qingdao Single\u2010cell Biotech. 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