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Conventional calibration techniques, however, require extensive datasets (typically exceeding 10\n                    <jats:sup>4<\/jats:sup>\n                    samples) to achieve statistical convergence in the presence of thermal noise and nonequilibrium artifacts. In this work, we employ the stochastic force inference (SFI) method, which combines information theory with statistical physics, to reconstruct deterministic force fields from noisy stochastic trajectories in optical traps. We demonstrate that the SFI\u2010based calibration offers exceptional advantages over traditional approaches, reducing the required acquisition duration by an order of magnitude while improving both accuracy and robustness. A femtonewton\u2010level force spectral sensitivity of 14.84 fN Hz\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    <jats:sup>\/<\/jats:sup>\n                    <jats:sup>2<\/jats:sup>\n                    was achieved in solution, along with a minimum detectable force of 3.56 \u00b1 0.96 fN, approaching the thermodynamic limit of optical force detection. We further applied the method to double\u2010stranded DNA (dsDNA) stretching experiments across low\u2010 and high\u2010force regimes, capturing signatures of both entropic bending rigidity and enthalpic stretching at a nanoscale resolution. Our results establish SFI as a data\u2010efficient and noise\u2010tolerant approach for optical tweezers calibration and molecular nanotechnology, with significant practical potential for biophysics and precision measurement.\n                  <\/jats:p>","DOI":"10.1002\/smtd.71064","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:33:02Z","timestamp":1790242382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Data\u2010Efficient, Precise Calibration of Optical Tweezers Using Stochastic Force Inference"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9349-3208","authenticated-orcid":false,"given":"Qiang","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8983-6063","authenticated-orcid":false,"given":"Yuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5594-7794","authenticated-orcid":false,"given":"Wenyu","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6616-5284","authenticated-orcid":false,"given":"Manman","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3983-7206","authenticated-orcid":false,"given":"Shaohui","family":"Yan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7447-1396","authenticated-orcid":false,"given":"Xianghua","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0382-4748","authenticated-orcid":false,"given":"Chen","family":"Bai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwei","family":"Min","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7132-0835","authenticated-orcid":false,"given":"Hongbao","family":"Xin","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Nanophotonic Manipulation Institute of Nanophotonics, College of Physics &amp; Optoelectronic Engineering Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohao","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1723-6680","authenticated-orcid":false,"given":"Baoli","family":"Yao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences  Xi'an China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586\u2010021\u201000062\u2010x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ade0073"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.07.034"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202002081"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1364\/PRJ.576337"},{"issue":"3","key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1021\/acsphotonics.4c00064","article-title":"Shining Light in Mechanobiology: Optical Tweezers, Scissors, and Beyond","volume":"11","author":"Stilgoe A. 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