{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:21:20Z","timestamp":1791098480355,"version":"4.1.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2009,1,15]],"date-time":"2009-01-15T00:00:00Z","timestamp":1231977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Harvard Stem Cell Institute Seed Grant"},{"DOI":"10.13039\/100000050","name":"NHLBI","doi-asserted-by":"crossref","award":["RO1HL090136"],"award-info":[{"award-number":["RO1HL090136"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In two separate articles published in this issue, Teisanu et al. and McQualter et al. report the use of flow cytometry and cell sorting to identify putative bronchiolar stem cells that are low in expression for the cell surface marker Sca-1 yet negative for CD34, and a mesenchymal, fibroblastic progenitor cell population from the lung that is positive for Sca-1, respectively. At first glance, these studies may seem to suggest that Sca-1 and CD34 are not markers of an epithelial stem cell population in the lung, as we previously determined in studies that identified bronchioalveolar stem cells (BASCs), and may also appear to contradict each other. However, here we point to evidence that the findings of these three studies are not mutually exclusive, and rather, that the different cell isolation and culturing protocols used in these studies have allowed for the identification of unique pulmonary cell populations. Rather than discounting previous work on BASCs, these studies reveal the existence of new methods and new cell types that will be interesting to use in future functional tests for their importance in lung biology and lung disease.<\/jats:p>","DOI":"10.1002\/stem.10","type":"journal-article","created":{"date-parts":[[2009,1,16]],"date-time":"2009-01-16T02:17:45Z","timestamp":1232072265000},"page":"606-611","source":"Crossref","is-referenced-by-count":32,"title":["Commentary: Sca-1 and Cells of the Lung: A matter of Different Sorts"],"prefix":"10.1093","volume":"27","author":[{"given":"David M.","family":"Raiser","sequence":"first","affiliation":[{"name":"Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA"},{"name":"Department of Genetics, Harvard Medical School, Harvard Stem Cell Institute, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla F.","family":"Kim","sequence":"additional","affiliation":[{"name":"Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA"},{"name":"Department of Genetics, Harvard Medical School, Harvard Stem Cell Institute, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,1,15]]},"reference":[{"key":"2022011221105005700_bib1","doi-asserted-by":"crossref","first-page":"14541","DOI":"10.1073\/pnas.261562798","article-title":"Flk-2 is a marker in hematopoietic stem cell differentiation: A simple method to isolate long-term stem cells","volume":"98","author":"Christensen","year":"2001","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022011221105005700_bib2","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1016\/j.cell.2005.05.026","article-title":"SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells","volume":"121","author":"Kiel","year":"2005","journal-title":"Cell"},{"key":"2022011221105005700_bib3","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1196\/annals.1392.021","article-title":"Dormant and self-renewing hematopoietic stem cells and their niches","volume":"1106","author":"Wilson","year":"2007","journal-title":"Ann N Y Acad Sci"},{"key":"2022011221105005700_bib4","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1016\/j.cell.2008.10.048","article-title":"Hematopoietic stem cells reversibly switch from dormancy to self-renewal during homeostasis and repair","volume":"135","author":"Wilson","year":"2008","journal-title":"Cell"},{"key":"2022011221105005700_bib5","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/nbt.1517","article-title":"Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells","volume":"27","author":"Foudi","year":"2008","journal-title":"Nat Biotechnol"},{"key":"2022011221105005700_bib6","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/nature04372","article-title":"Generation of a functional mammary gland from a single stem cell","volume":"439","author":"Shackleton","year":"2006","journal-title":"Nature"},{"issue":"Suppl 1","key":"2022011221105005700_bib7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1046\/j.1365-2184.36.s.1.3.x","article-title":"Isolation and characterization of functional mammary gland stem cells","volume":"36","author":"Welm","year":"2003","journal-title":"Cell Prolif"},{"key":"2022011221105005700_bib8","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1006\/dbio.2002.0625","article-title":"Sca-1(pos) cells in the mouse mammary gland represent an enriched progenitor cell population","volume":"245","author":"Welm","year":"2002","journal-title":"Dev Biol"},{"key":"2022011221105005700_bib9","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1165\/rcmb.2006-0386OC","article-title":"Isolation of an adult mouse lung mesenchymal progenitor cell population","volume":"37","author":"Summer","year":"2007","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2022011221105005700_bib10","doi-asserted-by":"crossref","first-page":"L97","DOI":"10.1152\/ajplung.00009.2003","article-title":"Side population cells and Bcrp1 expression in lung","volume":"285","author":"Summer","year":"2003","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022011221105005700_bib11","doi-asserted-by":"crossref","first-page":"L972","DOI":"10.1152\/ajplung.00090.2006","article-title":"Molecular and functional properties of lung SP cells","volume":"292","author":"Reynolds","year":"2007","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022011221105005700_bib12","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/j.cell.2005.03.032","article-title":"Identification of bronchioalveolar stem cells in normal lung and lung cancer","volume":"121","author":"Kim","year":"2005","journal-title":"Cell"},{"key":"2022011221105005700_bib13","doi-asserted-by":"crossref","first-page":"L624","DOI":"10.1152\/ajplung.00149.2003","article-title":"Molecular phenotype of airway side population cells","volume":"286","author":"Giangreco","year":"2004","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022011221105005700_bib14","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1084\/jem.183.4.1797","article-title":"Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo","volume":"183","author":"Goodell","year":"1996","journal-title":"J Exp Med"},{"key":"2022011221105005700_bib15","doi-asserted-by":"crossref","first-page":"11857","DOI":"10.1073\/pnas.0803574105","article-title":"Bmi1 is critical for lung tumorigenesis and bronchioalveolar stem cell expansion","volume":"105","author":"Dovey","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022011221105005700_bib16","doi-asserted-by":"crossref","first-page":"L1158","DOI":"10.1152\/ajplung.00298.2007","article-title":"Cellular kinetics and modeling of bronchioalveolar stem cell response during lung regeneration","volume":"294","author":"Nolen-Walston","year":"2008","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022011221105005700_bib17","doi-asserted-by":"crossref","first-page":"2929","DOI":"10.1172\/JCI31854","article-title":"Pten controls lung morphogenesis, bronchioalveolar stem cells, and onset of lung adenocarcinomas in mice","volume":"117","author":"Yanagi","year":"2007","journal-title":"J Clin Invest"},{"key":"2022011221105005700_bib18","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1038\/ng.157","article-title":"A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration","volume":"40","author":"Zhang","year":"2008","journal-title":"Nat Genet"},{"key":"2022011221105005700_bib19","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.bbrc.2008.10.052","article-title":"MicroRNA expression profile of bronchioalveolar stem cells from mouse lung","volume":"377","author":"Qian","year":"2008","journal-title":"Biochem Biophys Res Commun"},{"key":"2022011221105005700_bib20","doi-asserted-by":"crossref","first-page":"e2220","DOI":"10.1371\/journal.pone.0002220","article-title":"Phosphatidylinositol 3-kinase mediates bronchioalveolar stem cell expansion in mouse models of oncogenic K-ras-induced lung cancer","volume":"3","author":"Yang","year":"2008","journal-title":"Plos One"},{"key":"2022011221105005700_bib21","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1634\/stemcells.2008-0053","article-title":"Conditional stabilization of beta-catenin expands the pool of lung stem cells","volume":"26","author":"Reynolds","year":"2008","journal-title":"Stem Cells"},{"key":"2022011221105005700_bib22","doi-asserted-by":"crossref","DOI":"10.1101\/sqb.2008.73.036","article-title":"Stem cell biology in the lung and lung cancers: Employing pulmonary context and classic approaches","author":"Raiser","year":"2008","journal-title":"Cold Spring Harb Symp Quant Biol"},{"key":"2022011221105005700_bib23","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1634\/stemcells.2008-0838","article-title":"Prospective isolation of bronchiolar stem cells based upon immunophenotypic and autofluorescence characteristics","volume":"27","author":"Teisanu","year":"2009","journal-title":"Stem Cells"},{"key":"2022011221105005700_bib24","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1634\/stemcells.2008-0866","article-title":"Endogenous fibroblastic progenitor cells in the adult mouse lung are highly enriched in the Sca-1 positive fraction","volume":"27","author":"McQualter","year":"2009","journal-title":"Stem Cells"},{"key":"2022011221105005700_bib25","first-page":"975","article-title":"Developmental ontogeny of bronchio-alveolar stem cells","volume":"21","author":"Tyagi","year":"2007","journal-title":"FASEB J"},{"key":"2022011221105005700_bib26","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1002\/dvdy.21633","article-title":"Developmental alveolarization of the mouse lung","volume":"237","author":"Mund","year":"2008","journal-title":"Dev Dyn"},{"key":"2022011221105005700_bib27","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.cell.2004.08.012","article-title":"Self-renewal, multipotency, and the existence of two cell populations within an epithelial stem cell niche","volume":"118","author":"Blanpain","year":"2004","journal-title":"Cell"},{"key":"2022011221105005700_bib28","doi-asserted-by":"crossref","first-page":"6063","DOI":"10.1158\/0008-5472.CAN-06-0054","article-title":"Hedgehog signaling and Bmi-1 regulate self-renewal of normal and malignant human mammary stem cells","volume":"66","author":"Liu","year":"2006","journal-title":"Cancer Res"},{"key":"2022011221105005700_bib29","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1634\/stemcells.2008-0580","article-title":"Gli2 is a novel regulator of Sox2 expression in telencephalic neuroepithelial cells","volume":"27","author":"Takanaga","year":"2009","journal-title":"Stem Cells"},{"key":"2022011221105005700_bib30","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1242\/dev.00391","article-title":"A novel function for Hedgehog signalling in retinal pigment epithelium differentiation","volume":"130","author":"Perron","year":"2003","journal-title":"Development"},{"key":"2022011221105005700_bib31","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1126\/science.1072530","article-title":"\u201cStemness\u201d: Transcriptional profiling of embryonic and adult stem cells","volume":"298","author":"Ramalho-Santos","year":"2002","journal-title":"Science"},{"key":"2022011221105005700_bib32","doi-asserted-by":"crossref","first-page":"L1256","DOI":"10.1152\/ajplung.2000.278.6.L1256","article-title":"Conditional clara cell ablation reveals a self-renewing progenitor function of pulmonary neuroendocrine cells","volume":"278","author":"Reynolds","year":"2000","journal-title":"Am J Physiol Lung Cell Mol Physiol"}],"container-title":["Stem Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fstem.10","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/stem.10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/stem.10","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/stmcls\/article-pdf\/27\/3\/606\/41945750\/stmcls_27_3_606.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/stmcls\/article-pdf\/27\/3\/606\/41945750\/stmcls_27_3_606.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T02:46:31Z","timestamp":1642560391000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/stmcls\/article\/27\/3\/606-611\/6401908"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,15]]},"references-count":32,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2009,1,15]]},"published-print":{"date-parts":[[2009,3,1]]}},"URL":"https:\/\/doi.org\/10.1002\/stem.10","relation":{},"ISSN":["1066-5099","1549-4918"],"issn-type":[{"value":"1066-5099","type":"print"},{"value":"1549-4918","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2009,3,1]]},"published":{"date-parts":[[2009,1,15]]}}}