{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T10:36:41Z","timestamp":1791023801083,"version":"4.1.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2009,4,23]],"date-time":"2009-04-23T00:00:00Z","timestamp":1240444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"University of Bielefeld including a FiF grant from the University of Bielefeld"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Somatic neural and neural crest stem cells are promising sources for cellular therapy of several neurodegenerative diseases. However, because of practical considerations such as inadequate accessibility of the source material, the application of neural crest stem cells is strictly limited. The secondary palate is a highly regenerative and heavily innervated tissue, which develops embryonically under direct contribution of neural crest cells. Here, we describe for the first time the presence of nestin-positive neural crest-related stem cells within Meissner corpuscles and Merkel cell-neurite complexes located in the hard palate of adult Wistar rats. After isolation, palatal neural crest-related stem cells (pNC-SCs) were cultivated in the presence of epidermal growth factor and fibroblast growth factor under serum-free conditions, resulting in large amounts of neurospheres. We used immunocytochemical techniques and reverse transcriptase-polymerase chain reaction to assess the expression profile of pNC-SCs. In addition to the expression of neural crest stem cell markers such as Nestin, Sox2, and p75, we detected the expression of Klf4, Oct4, and c-Myc. pNC-SCs differentiated efficiently into neuronal and glial cells. Finally, we investigated the potential expression of stemness markers within the human palate. We identified expression of stem cell markers nestin and CD133 and the transcription factors needed for reprogramming of somatic cells into pluripotent cells: Sox2, Oct4, Klf4, and c-Myc. These data show that cells isolated from palatal rugae form neurospheres, are highly plastic, and express neural crest stem cell markers. In addition, pNC-SCs may have the ability to differentiate into functional neurons and glial cells, serving as a starting point for therapeutic studies.<\/jats:p>\n               <jats:p>Disclosure of potential conflicts of interest is found at the end of this article.<\/jats:p>","DOI":"10.1002\/stem.104","type":"journal-article","created":{"date-parts":[[2009,4,24]],"date-time":"2009-04-24T02:59:17Z","timestamp":1240541957000},"page":"1899-1910","source":"Crossref","is-referenced-by-count":134,"title":["Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells"],"prefix":"10.1093","volume":"27","author":[{"given":"Darius","family":"Widera","sequence":"first","affiliation":[{"name":"Institute of Cell Biology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christin","family":"Zander","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meike","family":"Heidbreder","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Kasperek","sequence":"additional","affiliation":[{"name":"Molecular Neurobiology Group, Faculty of Biology, University of Bielefeld, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Noll","sequence":"additional","affiliation":[{"name":"Institute of Cell Culture Technology, University of Bielefeld, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Seitz","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Mund-, Kiefer- und Plastische Gesichtschirurgie, Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belma","family":"Saldamli","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Mund-, Kiefer- und Plastische Gesichtschirurgie, Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Sudhoff","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Hals-, Nasen- und Ohrenheilkunde, Kopf- und Halschirurgie, Staedtische Kliniken Bielefeld, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Sader","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Mund-, Kiefer- und Plastische Gesichtschirurgie, Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Kaltschmidt","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Kaltschmidt","sequence":"additional","affiliation":[{"name":"Molecular Neurobiology Group, Faculty of Biology, University of Bielefeld, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,4,23]]},"reference":[{"key":"2022011221420704600_bib1","doi-asserted-by":"crossref","DOI":"10.5962\/bhl.title.104964","volume-title":"Untersuchungen \u00fcber die erste Anlage des Wirbeltierleibes. 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