{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:27:59Z","timestamp":1791271679289,"version":"4.1.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council U.K.","doi-asserted-by":"crossref","award":["G03000341"],"award-info":[{"award-number":["G03000341"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council U.K.","doi-asserted-by":"crossref","award":["MR\/J004553\/1"],"award-info":[{"award-number":["MR\/J004553\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Millers Trust"},{"name":"Great Ormond Street Hospital Children's Charity"},{"name":"Department of Health's National Institute for Health Research Biomedical Research Centre at Moorfields Eye Hospital"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Retinitis pigmentosa, other inherited retinal diseases, and age-related macular degeneration lead to untreatable blindness because of the loss of photoreceptors. We have recently shown that transplantation of mouse photoreceptors can result in improved vision. It is therefore timely to develop protocols for efficient derivation of photoreceptors from human pluripotent stem (hPS) cells. Current methods for photoreceptor derivation from hPS cells require long periods of culture and are rather inefficient. Here, we report that formation of a transient self-organized neuroepithelium from human embryonic stem cells cultured together with extracellular matrix is sufficient to induce a rapid conversion into retinal progenitors in 5 days. These retinal progenitors have the ability to differentiate very efficiently into Crx+ photoreceptor precursors after only 10 days and subsequently acquire rod photoreceptor identity within 4 weeks. Directed differentiation into photoreceptors using this protocol is also possible with human-induced pluripotent stem (hiPS) cells, facilitating the use of patient-specific hiPS cell lines for regenerative medicine and disease modeling.<\/jats:p>","DOI":"10.1002\/stem.1268","type":"journal-article","created":{"date-parts":[[2012,11,7]],"date-time":"2012-11-07T04:51:24Z","timestamp":1352263884000},"page":"408-414","source":"Crossref","is-referenced-by-count":75,"title":["Brief Report: Self-Organizing Neuroepithelium from Human Pluripotent Stem Cells Facilitates Derivation of Photoreceptors"],"prefix":"10.1093","volume":"31","author":[{"given":"C\u00e9dric","family":"Boucherie","sequence":"first","affiliation":[{"name":"Department of Genetics, UCL Institute of Ophthalmology, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayandip","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Molecular Immunology Unit and Centre for Immunodeficiency, UCL Institute of Child Health, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Els","family":"Henckaerts","sequence":"additional","affiliation":[{"name":"Division of Immunology, Infection and Inflammatory Diseases, King's College London School of Medicine, Guy's Hospital, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian J.","family":"Thrasher","sequence":"additional","affiliation":[{"name":"Molecular Immunology Unit and Centre for Immunodeficiency, UCL Institute of Child Health, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane C.","family":"Sowden","sequence":"additional","affiliation":[{"name":"Developmental Biology Unit, UCL Institute of Child Health, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin R.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Genetics, UCL 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