{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T05:30:35Z","timestamp":1771997435789,"version":"3.50.1"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T00:00:00Z","timestamp":1636588800000},"content-version":"vor","delay-in-days":2078,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Deciphering the extracellular signals that regulate SMC differentiation from stem cells is vital to further our understanding of the pathogenesis of vascular disease and for development of cell-based therapies and tissue engineering. Hyaluronan (HA) has emerged as an important component of the stem cell niche, however its role during stem cell differentiation is a complicated and inadequately defined process. This study aimed to investigate the role of HA in embryonic stem cell (ESC) differentiation toward a SMC lineage. ESCs were seeded on collagen-IV in differentiation medium to generate ESC-derived SMCs (esSMCs). Differentiation coincided with increased HA synthase (HAS) 2 expression, accumulation of extracellular HA and its assembly into pericellular matrices. Inhibition of HA synthesis by 4-methylumbelliferone (4MU), removal of the HA coat by hyaluronidase (HYAL) or HAS2 knockdown led to abrogation of SMC gene expression. HA activates ERK1\/2 and suppresses EGFR signaling pathways via its principle receptor, CD44. EGFR inactivation coincided with increased binding to CD44, which was further augmented by addition of high molecular weight (HMW)-HA either exogenously or via HAS2 overexpression through adenoviral gene transfer. HMW-HA-stimulated esSMCs displayed a functional role in vascular tissue engineering ex vivo, vasculogenesis in a matrigel plug model and SMC accumulation in neointimal lesions of vein grafts in mice. These findings demonstrate that HAS2-induced HA synthesis and organization drives ESC-SMC differentiation. Thus, remodeling of the HA microenvironment is a critical step in directing stem cell differentiation toward a vascular lineage, highlighting HA as a potential target for treatment of vascular diseases.<\/jats:p>","DOI":"10.1002\/stem.2328","type":"journal-article","created":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T13:52:06Z","timestamp":1455198726000},"page":"1225-1238","source":"Crossref","is-referenced-by-count":42,"title":["Hyaluronan Is Crucial for Stem Cell Differentiation into Smooth Muscle Lineage"],"prefix":"10.1093","volume":"34","author":[{"given":"Russell M.L.","family":"Simpson","sequence":"first","affiliation":[{"name":"Cardiovascular Division, BHF Centre for Vascular Regeneration, King's College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuechong","family":"Hong","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, BHF Centre for Vascular Regeneration, King's College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei Mei","family":"Wong","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, BHF Centre for Vascular Regeneration, King's College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eirini","family":"Karamariti","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, BHF Centre for Vascular Regeneration, King's College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirin Issa","family":"Bhaloo","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, BHF Centre for Vascular Regeneration, King's College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek","family":"Warren","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, BHF Centre for Vascular Regeneration, King's College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhua","family":"Hu","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, BHF Centre for Vascular Regeneration, King's College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingbo","family":"Xu","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, BHF Centre for Vascular Regeneration, King's College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,3,4]]},"reference":[{"key":"2022010809592462700_stem2328-bib-0001","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/292154a0","article-title":"Establishment in culture of pluripotential cells from mouse embryos","volume":"292","author":"Evans","year":"1981","journal-title":"Nature"},{"key":"2022010809592462700_stem2328-bib-0002","doi-asserted-by":"crossref","first-page":"7634","DOI":"10.1073\/pnas.78.12.7634","article-title":"Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells","volume":"78","author":"Martin","year":"1981","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022010809592462700_stem2328-bib-0003","doi-asserted-by":"crossref","first-page":"C1560","DOI":"10.1152\/ajpcell.00221.2004","article-title":"Transforming growth factor-beta1 signaling contributes to development of smooth muscle cells from embryonic stem cells","volume":"287","author":"Sinha","year":"2004","journal-title":"Am J Physiol Cell Physiol"},{"key":"2022010809592462700_stem2328-bib-0004","doi-asserted-by":"crossref","first-page":"C342","DOI":"10.1152\/ajpcell.00341.2006","article-title":"Stem cell-derived Sca-1\u2009+\u2009progenitors differentiate into smooth muscle cells, which is mediated by collagen IV-integrin alpha1\/beta1\/alphav and PDGF receptor pathways","volume":"292","author":"Xiao","year":"2007","journal-title":"Am J Physiol Cell Physiol"},{"key":"2022010809592462700_stem2328-bib-0005","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.ceb.2012.07.001","article-title":"Extracellular matrix, integrins, and growth factors as tailors of the stem cell niche","volume":"24","author":"Brizzi","year":"2012","journal-title":"Curr Opin Cell Biol"},{"key":"2022010809592462700_stem2328-bib-0006","doi-asserted-by":"crossref","first-page":"e26029","DOI":"10.1371\/journal.pone.0026029","article-title":"Uniaxial mechanical strain modulates the differentiation of neural crest stem cells into smooth muscle lineage on micropatterned surfaces","volume":"6","author":"Li","year":"2011","journal-title":"PloS one"},{"key":"2022010809592462700_stem2328-bib-0007","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1038\/nrm3620","article-title":"Role of the extracellular matrix in regulating stem cell fate","volume":"14","author":"Watt","year":"2013","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2022010809592462700_stem2328-bib-0008","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.biocel.2013.12.016","article-title":"Extensive CD44-dependent hyaluronan coats on human bone marrow-derived mesenchymal stem cells produced by hyaluronan synthases HAS1, HAS2 and HAS3","volume":"48","author":"Qu","year":"2014","journal-title":"Int J Biochem Cell Biol"},{"key":"2022010809592462700_stem2328-bib-0009","doi-asserted-by":"crossref","first-page":"3045","DOI":"10.1634\/stemcells.2007-0296","article-title":"Putative role of hyaluronan and its related genes, HAS2 and RHAMM, in human early preimplantation embryogenesis and embryonic stem cell characterization","volume":"25","author":"Choudhary","year":"2007","journal-title":"Stem Cells"},{"key":"2022010809592462700_stem2328-bib-0010","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1182\/blood-2002-01-0030","article-title":"Characterization of hyaluronan synthase expression and hyaluronan synthesis in bone marrow mesenchymal progenitor cells: Predominant expression of HAS1 mRNA and up-regulated hyaluronan synthesis in bone marrow cells derived from multiple myeloma patients","volume":"100","author":"Calabro","year":"2002","journal-title":"Blood"},{"key":"2022010809592462700_stem2328-bib-0011","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1182\/blood-2002-05-1344","article-title":"Hyaluronan is synthesized by primitive hemopoietic cells, participates in their lodgment at the endosteum following transplantation, and is involved in the regulation of their proliferation and differentiation in vitro","volume":"101","author":"Nilsson","year":"2003","journal-title":"Blood"},{"key":"2022010809592462700_stem2328-bib-0012","doi-asserted-by":"crossref","first-page":"2918","DOI":"10.1182\/blood.V93.9.2918","article-title":"Potential role for hyaluronan and the hyaluronan receptor RHAMM in mobilization and trafficking of hematopoietic progenitor cells","volume":"93","author":"Pilarski","year":"1999","journal-title":"Blood"},{"key":"2022010809592462700_stem2328-bib-0013","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1089\/ten.tea.2008.0067","article-title":"Influence of three-dimensional hyaluronic acid microenvironments on mesenchymal stem cell chondrogenesis","volume":"15","author":"Chung","year":"2009","journal-title":"Tissue Eng Part A"},{"key":"2022010809592462700_stem2328-bib-0014","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1074\/jbc.273.4.1923","article-title":"Characterization and molecular evolution of a vertebrate hyaluronan synthase gene family","volume":"273","author":"Spicer","year":"1998","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0015","doi-asserted-by":"crossref","first-page":"25117","DOI":"10.1074\/jbc.273.39.25117","article-title":"Molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes","volume":"273","author":"Spicer","year":"1998","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0016","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1369\/jhc.2009.954826","article-title":"Synthesis and organization of hyaluronan and versican by embryonic stem cells undergoing embryoid body differentiation","volume":"58","author":"Shukla","year":"2010","journal-title":"J Histochem Cytochem"},{"key":"2022010809592462700_stem2328-bib-0017","doi-asserted-by":"crossref","first-page":"45801","DOI":"10.1074\/jbc.M308168200","article-title":"Elevated hyaluronan production induces mesenchymal and transformed properties in epithelial cells","volume":"278","author":"Zoltan-Jones","year":"2003","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0018","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.2353\/ajpath.2010.090802","article-title":"Aging fibroblasts resist phenotypic maturation because of impaired hyaluronan-dependent CD44\/epidermal growth factor receptor signaling","volume":"176","author":"Simpson","year":"2010","journal-title":"Am J Pathol"},{"key":"2022010809592462700_stem2328-bib-0019","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.2353\/ajpath.2009.090045","article-title":"Age-related changes in pericellular hyaluronan organization leads to impaired dermal fibroblast to myofibroblast differentiation","volume":"175","author":"Simpson","year":"2009","journal-title":"Am J Pathol"},{"key":"2022010809592462700_stem2328-bib-0020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bbrc.2007.04.211","article-title":"Hyaluronan preserves the proliferation and differentiation potentials of long-term cultured murine adipose-derived stromal cells","volume":"360","author":"Chen","year":"2007","journal-title":"Biochem Biophys Res Commun"},{"key":"2022010809592462700_stem2328-bib-0021","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.1073\/pnas.052026799","article-title":"Abnormal accumulation of hyaluronan matrix diminishes contact inhibition of cell growth and promotes cell migration","volume":"99","author":"Itano","year":"2002","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022010809592462700_stem2328-bib-0022","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1111\/j.1523-1755.2004.00457.x","article-title":"Hyaluronan and proximal tubular cell migration","volume":"65","author":"Ito","year":"2004","journal-title":"Kidney Int"},{"key":"2022010809592462700_stem2328-bib-0023","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1038\/ncb797","article-title":"A novel PKC-regulated mechanism controls CD44 ezrin association and directional cell motility","volume":"4","author":"Legg","year":"2002","journal-title":"Nat Cell Biol"},{"key":"2022010809592462700_stem2328-bib-0024","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1042\/bj2390445","article-title":"Increased hyaluronate synthesis is required for fibroblast detachment and mitosis","volume":"239","author":"Brecht","year":"1986","journal-title":"Biochem J"},{"key":"2022010809592462700_stem2328-bib-0025","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1161\/01.ATV.19.4.1004","article-title":"Formation of hyaluronan- and versican-rich pericellular matrix is required for proliferation and migration of vascular smooth muscle cells","volume":"19","author":"Evanko","year":"1999","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"2022010809592462700_stem2328-bib-0026","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1002\/jcp.10288","article-title":"Modulation of human fibroblast gap junction intercellular communication by hyaluronan","volume":"196","author":"Moyer","year":"2003","journal-title":"J Cell Physiol"},{"key":"2022010809592462700_stem2328-bib-0027","doi-asserted-by":"crossref","first-page":"563818","DOI":"10.1155\/2015\/563818","article-title":"Size matters: Molecular weight specificity of hyaluronan effects in cell biology","volume":"2015","author":"Cyphert","year":"2015","journal-title":"Int J Cell Biol"},{"key":"2022010809592462700_stem2328-bib-0028","doi-asserted-by":"crossref","first-page":"18679","DOI":"10.1074\/jbc.M313178200","article-title":"Selective expression and functional characteristics of three mammalian hyaluronan synthases in oncogenic malignant transformation","volume":"279","author":"Itano","year":"2004","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0029","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1080\/15216540214929","article-title":"Mammalian hyaluronan synthases","volume":"54","author":"Itano","year":"2002","journal-title":"IUBMB Life"},{"key":"2022010809592462700_stem2328-bib-0030","doi-asserted-by":"crossref","first-page":"25085","DOI":"10.1074\/jbc.274.35.25085","article-title":"Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties","volume":"274","author":"Itano","year":"1999","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0031","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1042\/bst0270109","article-title":"Mammalian hyaluronan synthases: Investigation of functional relationships in vivo","volume":"27","author":"Spicer","year":"1999","journal-title":"Biochem Soc Trans"},{"key":"2022010809592462700_stem2328-bib-0032","doi-asserted-by":"crossref","first-page":"36777","DOI":"10.1074\/jbc.R700036200","article-title":"Hyaluronan synthases: A decade-plus of novel glycosyltransferases","volume":"282","author":"Weigel","year":"2007","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0033","doi-asserted-by":"crossref","first-page":"346972","DOI":"10.1155\/2012\/346972","article-title":"Hyaluronan regulates cell behavior: A potential niche matrix for stem cells","volume":"2012","author":"Solis","year":"2012","journal-title":"Biochem Res Int"},{"key":"2022010809592462700_stem2328-bib-0034","first-page":"9","article-title":"Hyaluronan is required for generation of hematopoietic cells during differentiation of human embryonic stem cells","volume":"5","author":"Schraufstatter","year":"2010","journal-title":"J Stem Cells"},{"key":"2022010809592462700_stem2328-bib-0035","doi-asserted-by":"crossref","first-page":"11298","DOI":"10.1073\/pnas.0703723104","article-title":"Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells","volume":"104","author":"Gerecht","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022010809592462700_stem2328-bib-0036","doi-asserted-by":"crossref","first-page":"2706","DOI":"10.1016\/j.biomaterials.2007.01.021","article-title":"Bioactive hydrogel scaffolds for controllable vascular differentiation of human embryonic stem cells","volume":"28","author":"Ferreira","year":"2007","journal-title":"Biomaterials"},{"key":"2022010809592462700_stem2328-bib-0037","doi-asserted-by":"crossref","first-page":"2789","DOI":"10.1016\/j.biomaterials.2003.09.054","article-title":"Stimulation of in vivo angiogenesis by cytokine-loaded hyaluronic acid hydrogel implants","volume":"25","author":"Peattie","year":"2004","journal-title":"Biomaterials"},{"key":"2022010809592462700_stem2328-bib-0038","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1016\/j.actbio.2013.07.025","article-title":"Design of cell-matrix interactions in hyaluronic acid hydrogel scaffolds","volume":"10","author":"Lam","year":"2014","journal-title":"Acta Biomater"},{"key":"2022010809592462700_stem2328-bib-0039","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1016\/S0002-9440(10)65675-1","article-title":"Mouse model of venous bypass graft arteriosclerosis","volume":"153","author":"Zou","year":"1998","journal-title":"Am J Pathol"},{"key":"2022010809592462700_stem2328-bib-0040","doi-asserted-by":"crossref","first-page":"33281","DOI":"10.1074\/jbc.M405918200","article-title":"A novel mechanism for the inhibition of hyaluronan biosynthesis by 4-methylumbelliferone","volume":"279","author":"Kakizaki","year":"2004","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0041","doi-asserted-by":"crossref","first-page":"14026","DOI":"10.1074\/jbc.M507734200","article-title":"Hyaluronan-CD44 interaction with leukemia-associated RhoGEF and epidermal growth factor receptor promotes Rho\/Ras co-activation, phospholipase C epsilon-Ca2\u2009+\u2009signaling, and cytoskeleton modification in head and neck squamous cell carcinoma cells","volume":"281","author":"Bourguignon","year":"2006","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0042","doi-asserted-by":"crossref","first-page":"e58760","DOI":"10.1371\/journal.pone.0058760","article-title":"Crucial role of hyaluronan in neointimal formation after vascular injury","volume":"8","author":"Kashima","year":"2013","journal-title":"PloS one"},{"key":"2022010809592462700_stem2328-bib-0043","doi-asserted-by":"crossref","first-page":"17618","DOI":"10.1074\/jbc.M111.226563","article-title":"Hyaluronan facilitates transforming growth factor-beta1-dependent proliferation via CD44 and epidermal growth factor receptor interaction","volume":"286","author":"Meran","year":"2011","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0044","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1083\/jcb.200605113","article-title":"HDAC3 is crucial in shear- and VEGF-induced stem cell differentiation toward endothelial cells","volume":"174","author":"Zeng","year":"2006","journal-title":"J Cell Biol"},{"key":"2022010809592462700_stem2328-bib-0045","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/s00441-009-0780-3","article-title":"Hyaluronan substratum induces multidrug resistance in human mesenchymal stem cells via CD44 signaling","volume":"336","author":"Liu","year":"2009","journal-title":"Cell Tissue Res"},{"key":"2022010809592462700_stem2328-bib-0046","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/s00441-008-0699-0","article-title":"Hyaluronan substratum holds mesenchymal stem cells in slow-cycling mode by prolonging G1 phase","volume":"334","author":"Liu","year":"2008","journal-title":"Cell Tissue Res"},{"key":"2022010809592462700_stem2328-bib-0047","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.matbio.2011.01.002","article-title":"Reduced chondrogenic matrix accumulation by 4-methylumbelliferone reveals the potential for selective targeting of UDP-glucose dehydrogenase","volume":"30","author":"Clarkin","year":"2011","journal-title":"Matrix Biol"},{"key":"2022010809592462700_stem2328-bib-0048","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1177\/43.7.7608523","article-title":"Association of hyaluronan with rat vascular endothelial and smooth muscle cells","volume":"43","author":"Eggli","year":"1995","journal-title":"J Histochem Cytochem"},{"key":"2022010809592462700_stem2328-bib-0049","doi-asserted-by":"crossref","first-page":"36721","DOI":"10.1074\/jbc.M110.162305","article-title":"Hyaluronan-CD44 interaction promotes c-Src-mediated twist signaling, microRNA-10b expression, and RhoA\/RhoC up-regulation, leading to Rho-kinase-associated cytoskeleton activation and breast tumor cell invasion","volume":"285","author":"Bourguignon","year":"2010","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0050","doi-asserted-by":"crossref","first-page":"17635","DOI":"10.1074\/jbc.M800109200","article-title":"Hyaluronan-CD44 interaction activates stem cell marker Nanog, Stat-3-mediated MDR1 gene expression, and ankyrin-regulated multidrug efflux in breast and ovarian tumor cells","volume":"283","author":"Bourguignon","year":"2008","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0051","doi-asserted-by":"crossref","first-page":"35111","DOI":"10.1074\/jbc.M103481200","article-title":"Hyaluronan enters keratinocytes by a novel endocytic route for catabolism","volume":"276","author":"Tammi","year":"2001","journal-title":"J Biol Chem"},{"key":"2022010809592462700_stem2328-bib-0052","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1177\/002215549904701013","article-title":"Intracellular localization of hyaluronan in proliferating cells","volume":"47","author":"Evanko","year":"1999","journal-title":"J Histochem Cytochem"},{"key":"2022010809592462700_stem2328-bib-0053","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1084\/jem.181.2.475","article-title":"The development of T and non-T cell lineages from CD34\u2009+\u2009human thymic precursors can be traced by the differential expression of CD44","volume":"181","author":"Marquez","year":"1995","journal-title":"J Exp Med"},{"key":"2022010809592462700_stem2328-bib-0054","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1038\/nrc1391","article-title":"Hyaluronan: From extracellular glue to pericellular cue","volume":"4","author":"Toole","year":"2004","journal-title":"Nat Rev Cancer"},{"key":"2022010809592462700_stem2328-bib-0055","doi-asserted-by":"crossref","first-page":"e48523","DOI":"10.1371\/journal.pone.0048523","article-title":"Inhibition of hyaluronan synthesis reduces versican and fibronectin levels in trabecular meshwork cells","volume":"7","author":"Keller","year":"2012","journal-title":"PloS One"},{"key":"2022010809592462700_stem2328-bib-0056","doi-asserted-by":"crossref","first-page":"e32459","DOI":"10.1371\/journal.pone.0032459","article-title":"EGFR kinase promotes acquisition of stem cell-like properties: A potential therapeutic target in head and neck squamous cell carcinoma stem cells","volume":"7","author":"Abhold","year":"2012","journal-title":"PloS one"},{"key":"2022010809592462700_stem2328-bib-0057","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1006\/dbio.1999.9265","article-title":"Suppression of SHP-2 and ERK signalling promotes self-renewal of mouse embryonic stem cells","volume":"210","author":"Burdon","year":"1999","journal-title":"Dev Biol"},{"key":"2022010809592462700_stem2328-bib-0058","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1038\/sj.jid.5701098","article-title":"All-trans retinoic acid-induced hyaluronan production and hyperplasia are partly mediated by EGFR signaling in epidermal keratinocytes","volume":"128","author":"Pasonen-Seppanen","year":"2008","journal-title":"J Invest Dermatol"},{"key":"2022010809592462700_stem2328-bib-0059","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1038\/ncomms1867","article-title":"Differentiation of multipotent vascular stem cells contributes to vascular diseases","volume":"3","author":"Tang","year":"2012","journal-title":"Nat Commun"},{"key":"2022010809592462700_stem2328-bib-0060","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.1161\/ATVBAHA.113.300902","article-title":"Adventitial stem cells in vein grafts display multilineage potential that contributes to neointimal formation","volume":"33","author":"Chen","year":"2013","journal-title":"Arterioscler Thromb Vasc Biol"}],"container-title":["Stem Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fstem.2328","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/stem.2328","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/stem.2328","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/stmcls\/article-pdf\/34\/5\/1225\/42028566\/stmcls_34_5_1225.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/stmcls\/article-pdf\/34\/5\/1225\/42028566\/stmcls_34_5_1225.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T02:57:46Z","timestamp":1642561066000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/stmcls\/article\/34\/5\/1225-1238\/6408161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,4]]},"references-count":60,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2016,3,4]]},"published-print":{"date-parts":[[2016,5,1]]}},"URL":"https:\/\/doi.org\/10.1002\/stem.2328","relation":{},"ISSN":["1066-5099","1549-4918"],"issn-type":[{"value":"1066-5099","type":"print"},{"value":"1549-4918","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2016,5,1]]},"published":{"date-parts":[[2016,3,4]]}}}