{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:46:57Z","timestamp":1791654417104,"version":"4.3.4"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2017,2,5]],"date-time":"2017-02-05T00:00:00Z","timestamp":1486252800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"National Institutes of Health National Heart Lung Blood Institute","award":["NHLBI grant PO1 HL107146"],"award-info":[{"award-number":["NHLBI grant PO1 HL107146"]}]},{"name":"\u201cMiguel Servet\u201d tenure track program","award":["CP10\/00438"],"award-info":[{"award-number":["CP10\/00438"]}]},{"name":"Fondo de Investigacio'n Sanitaria"},{"name":"Spanish Ministry of Economy and Competitiveness-European Regional Development Fund","award":["SAF2012-36186"],"award-info":[{"award-number":["SAF2012-36186"]}]},{"name":"Spanish Ministry of Economy and Competitiveness-European Regional Development Fund","award":["SAF2015-65019-R"],"award-info":[{"award-number":["SAF2015-65019-R"]}]},{"DOI":"10.13039\/501100008530","name":"ERDF","doi-asserted-by":"publisher","award":["PI11\/0085"],"award-info":[{"award-number":["PI11\/0085"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"ERDF","doi-asserted-by":"publisher","award":["PI14\/00228"],"award-info":[{"award-number":["PI14\/00228"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Spanish Biomedical Research Center in Diabetes and Associated Metabolic Disorders","award":["CB07708\/0012"],"award-info":[{"award-number":["CB07708\/0012"]}]},{"name":"Ministerio de Salud Carlos III","award":["CD10\/00285"],"award-info":[{"award-number":["CD10\/00285"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The clinical effectiveness of systemically administered human mesenchymal stem cells (hMSCs) depends on their capacity to engage vascular endothelium. hMSCs derived from bone marrow (BM-hMSCs) natively lack endothelial binding capacity, but express a CD44 glycovariant containing N-linked sialyllactosamines that can be \u03b1(1,3)-fucosylated using fucosyltransferase-VI (FTVI) to enforce sLeX decorations, thereby creating hematopoietic cell E-\/L-selectin ligand (HCELL). HCELL expression programs potent shear-resistant adhesion of circulating cells to endothelial beds expressing E-selectin. An alternative source of hMSCs is adipose tissue (A-hMSCs), and we assessed whether A-hMSCs bind E-selectin and\/or possess sialyllactosamine-decorated CD44 accessible to \u03b1(1,3)-fucosylation. Similar to BM-hMSCs, we found that A-hMSCs natively lack E-selectin ligands, but FTVI-mediated cell surface \u03b1(1,3)-fucosylation induces sLeX expression and robust E-selectin binding secondary to conversion of CD44 into HCELL. Moreover, treatment with the \u03b1(1,3)-fucosyltransferase-FTVII also generated expression of HCELL on both BM-hMSCs and A-hMSCs, with sLeX decorations created on N-linked glycans of the \u201cstandard\u201d CD44 (CD44s) isoform. The finding that hMSCs from both source tissues each lack native E-selectin ligand expression prompted examination of the expression of glycosyltransferases that direct lactosaminyl glycan synthesis. These studies reveal that both types of hMSCs conspicuously lack transcripts encoding \u03b1(1,3)-fucosyltransferases, but equally express glycosyltransferases critical to creation of sialyllactosamines. Collectively, these data indicate that assembly of a sialyllactosaminyl-decorated CD44s glycovariant is a conserved feature of hMSCs derived from adipose tissue and marrow, thus identifying a CD44 glycosignature of these cells and supporting the applicability of cell surface \u03b1(1,3)-fucosylation in programming migration of systemically administered A-hMSCs to sites of tissue injury\/inflammation.<\/jats:p>","DOI":"10.1002\/stem.2549","type":"journal-article","created":{"date-parts":[[2016,11,26]],"date-time":"2016-11-26T10:52:42Z","timestamp":1480157562000},"page":"1080-1092","source":"Crossref","is-referenced-by-count":38,"title":["A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells"],"prefix":"10.1093","volume":"35","author":[{"given":"Gisela","family":"Pach\u00f3n-Pe\u00f1a","sequence":"first","affiliation":[{"name":"a Department of Dermatology, and Harvard Skin Disease Research Center, Brigham and Women's Hospital, Boston, Massachusetts, USA"},{"name":"b Program of Excellence in Glycosciences, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conor","family":"Donnelly","sequence":"additional","affiliation":[{"name":"a Department of Dermatology, and Harvard Skin Disease Research Center, Brigham and Women's Hospital, Boston, Massachusetts, USA"},{"name":"b Program of Excellence in Glycosciences, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catalina","family":"Ruiz-Ca\u00f1ada","sequence":"additional","affiliation":[{"name":"a Department of Dermatology, and Harvard Skin Disease Research Center, Brigham and Women's Hospital, Boston, Massachusetts, USA"},{"name":"b Program of Excellence in Glycosciences, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Katz","sequence":"additional","affiliation":[{"name":"c Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Florida College of Medicine, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Fern\u00e1ndez-Veledo","sequence":"additional","affiliation":[{"name":"d Hospital Universitario de Tarragona Joan XXIII. Institut d'Investigaci\u00f3 Sanit\u00e0ria Pere Virgili Universitat Rovira i Virgili, Tarragona, Spain"},{"name":"e CIBER de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan","family":"Vendrell","sequence":"additional","affiliation":[{"name":"d Hospital Universitario de Tarragona Joan XXIII. Institut d'Investigaci\u00f3 Sanit\u00e0ria Pere Virgili Universitat Rovira i Virgili, Tarragona, Spain"},{"name":"e CIBER de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Sackstein","sequence":"additional","affiliation":[{"name":"a Department of Dermatology, and Harvard Skin Disease Research Center, Brigham and Women's Hospital, Boston, Massachusetts, USA"},{"name":"b Program of Excellence in Glycosciences, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"f Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,2,5]]},"reference":[{"key":"2022022509204782000_stem2549-bib-0001","doi-asserted-by":"crossref","first-page":"3838","DOI":"10.1182\/blood.V99.10.3838","article-title":"Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli","volume":"10","author":"Di Nicola","year":"2002","journal-title":"Blood"},{"key":"2022022509204782000_stem2549-bib-0002","doi-asserted-by":"crossref","first-page":"4619","DOI":"10.1182\/blood-2003-11-3909","article-title":"Human bone marrow stromal cells inhibit allogeneic T-cell responses by indoleamine 2,3-dioxygenase-mediated tryptophan degradation","volume":"12","author":"Meisel","year":"2004","journal-title":"Blood"},{"key":"2022022509204782000_stem2549-bib-0003","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1002\/jcp.22408","article-title":"Stromal stem cells from adipose tissue and bone marrow of age-matched female donors display distinct immunophenotypic profiles","volume":"3","author":"Pach\u00f3n-Pe\u00f1a","year":"2011","journal-title":"J Cell Physiol"},{"key":"2022022509204782000_stem2549-bib-0004","doi-asserted-by":"crossref","first-page":"464","DOI":"10.5966\/sctm.2015-0161","article-title":"Obesity determines the immunophenotypic profile and functional characteristics of human mesenchymal stem cells from adipose tissue.","volume":"5","author":"Pach\u00f3n-Pe\u00f1a","year":"2016","journal-title":"Stem Cells Transl Med"},{"key":"2022022509204782000_stem2549-bib-0005","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.jcyt.2013.02.006","article-title":"Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal\/stem cells: A joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT)","volume":"15","author":"Bourin","year":"2013","journal-title":"Cytotherapy"},{"key":"2022022509204782000_stem2549-bib-0006","first-page":"1073","article-title":"Unveiling the differences of secretome of human bone marrow mesenchymal stem cells, adipose tissue-derived stem cells, and human umbilical cord perivascular cells: A proteomic analysis. 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