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In current protocols, hPSCs are traditionally differentiated into an obligate rostro-dorsal ectodermal fate expressing PAX6 after 6 to 12\u2009days in\u2009vitro when protected from mesendoderm inducers. This rate-limiting step has performed a long-standing role in hindering the development of rapid differentiation protocols for ectoderm-derived cell types, as any protocol requires 6 to 10\u2009days in\u2009vitro to simply initiate. Here, we report efficient differentiation of hPSCs into a naive early ectodermal intermediate within 24\u2009hours using combined inhibition of bone morphogenic protein and fibroblast growth factor signaling. The induced population responds immediately to morphogen gradients to upregulate rostro-caudal neurodevelopmental landmark gene expression in a generally accelerated fashion. This method can serve as a new platform for the development of novel, rapid, and efficient protocols for the manufacture of hPSC-derived neural lineages.<\/jats:p><jats:sec><jats:title\/><\/jats:sec>","DOI":"10.1002\/stem.3260","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T18:07:55Z","timestamp":1596478075000},"page":"1400-1408","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2778-9019","authenticated-orcid":false,"given":"Patrick","family":"Walsh","sequence":"first","affiliation":[{"name":"1 Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA"},{"name":"2 Department of Genetics, Cell Biology and Development, University of Minnesota, 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