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Both food restriction and deprivation alter protein and mRNA levels of opioid peptides and receptors. Antisera directed against Exon 4 of the MOR\u20101\u2010like immunoreactivity (LI) (Exon 4) clone or directed against mouse Exons 7\/8 (<jats:italic>mE7\/8\u2010LI<\/jats:italic>) revealed high levels of immunoreactivity in brain nuclei related to feeding behavior. Therefore, the present study assessed MOR\u20101LI and mE7\/8\u2010LI in hypothalamic and extrahypothalamic sites in rats exposed to ad libitum feeding, food restriction (2, 7, 14 days), or food deprivation (24, 48 h). MOR\u20101\u2010LI displayed robust reactivity, but was insensitive to food restriction or deprivation. mE7\/8\u2010LI, both in terms of cell counts and relative optical density, was significantly and selectively increased in the dorsal and ventral parvocellular subdivisions of the hypothalamic paraventricular nucleus in food\u2010restricted (14 days) rats, but all other restriction or deprivation regimens were ineffective in other hypothalamic nuclei. In contrast, significant and site\u2010specific decreases in relative optical density in the rostral part of the nucleus tractus solitarius (NTS) were observed in food\u2010restricted (2, 7 days) or food\u2010deprived (24, 48 h) animals, but these regimens were ineffective in the other extrahypothalamic sites. This study indicates the sensitivity of this mE7\/8\u2010LI probe in the hypothalamic parvocellular paraventricular nucleus and rostral NTS to food restriction and deprivation in rats. Synapse 63:585\u2013597, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/syn.20639","type":"journal-article","created":{"date-parts":[[2009,3,19]],"date-time":"2009-03-19T15:57:22Z","timestamp":1237478242000},"page":"585-597","source":"Crossref","is-referenced-by-count":6,"title":["Changes in mouse mu opioid receptor Exon 7\/8\u2010like immunoreactivity following food restriction and food deprivation in rats"],"prefix":"10.1002","volume":"63","author":[{"given":"Maria M.","family":"Hadjimarkou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Abbadie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lora 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