{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:27:13Z","timestamp":1762079233049,"version":"3.37.3"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,12,3]],"date-time":"2010-12-03T00:00:00Z","timestamp":1291334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Synapse"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Depletion of central serotonin (5\u2010HT) levels and dysfunction in serotonergic transmission are implicated in a variety of human CNS disorders. The mechanisms behind these serotonergic deficits have been widely studied using rodent models, but only to a limited extent in larger animal models. The pig is increasingly used as an experimental animal model especially in neuroscience research. Here, we present an approach for serotonin depletion in the pig brain. Central serotonin depletion in Danish Landrace pigs was achieved following 4 days treatment with<jats:italic>para<\/jats:italic>\u2010chlorophenylalanine (<jats:italic>p<\/jats:italic>CPA). On day 5, tissue concentrations of 5\u2010HT in seven distinct brain structures from one hemisphere: frontal and occipital cortex, striatum, hippocampus, cerebellum, rostral, and caudal brain stem, were determined. The other hemisphere was processed for receptor autoradiography. Treatments with 50 mg\/kg and 100 mg\/kg<jats:italic>p<\/jats:italic>CPA caused average decreases in 5\u2010HT concentrations of 61% \u00b1 14% and 66% \u00b1 16%, respectively, and a substantial loss of 5\u2010HT immunostaining was seen throughout the brain. The serotonin depletion significantly increased 5\u2010HT<jats:sub>4<\/jats:sub>receptor binding in nucleus accumbens, but did not alter 5\u2010HT<jats:sub>1A<\/jats:sub>and 5\u2010HT<jats:sub>2A<\/jats:sub>receptor or serotonin transporter binding in any brain region. In conclusion, 4 days treatment with<jats:italic>p<\/jats:italic>CPA effectively reduces 5\u2010HT levels in the pig brain. Further, whereas several 5\u2010HT markers did not change after the<jats:italic>p<\/jats:italic>CPA treatment, 5\u2010HT<jats:sub>4<\/jats:sub>receptors were consistently upregulated, indicating a greater susceptibility of this receptor to altered 5\u2010HT levels. This porcine model of serotonin depletion will be useful in future studies of cerebral serotonergic dysfunction. Synapse 2011. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/syn.20827","type":"journal-article","created":{"date-parts":[[2010,6,17]],"date-time":"2010-06-17T03:58:58Z","timestamp":1276747138000},"page":"136-145","source":"Crossref","is-referenced-by-count":18,"title":["An approach for serotonin depletion in pigs: Effects on serotonin receptor binding"],"prefix":"10.1002","volume":"65","author":[{"given":"Anders","family":"Ettrup","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgitte R.","family":"Kornum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pia","family":"Weikop","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gitte 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