{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:40:38Z","timestamp":1761864038658,"version":"3.41.2"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,11,9]],"date-time":"2019-11-09T00:00:00Z","timestamp":1573257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Synapse"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We aimed to compare the performance of acellular nerves prepared by different decellularization methods, screening out the optimal decellularization protocol, repairing the sciatic nerve defects in rats by the allogeneic transplantation, and evaluating the effect of regenerative nerve on the function reconstruction. The Sondell, SB\u2010SDS, TnBP, and the high\/low permeation methods were used to decellularize donor nerves. Nerves without any treatment were as the control group. The histological results were evaluated by HE staining and toluidine blue (TB) staining. The proliferation activity of L929 cells was detected by CCK\u20108 assay. The adhesion of Schwann cells was observed and quantified by <jats:italic>SEM<\/jats:italic>. Balb\/c mice were used to evaluate the cellular and humoral immunogenicity of the nerve scaffolds. The rat sciatic nerve defect model was applied to observe the repair effect of acellular nerve scaffold in vivo. To SB\u2010SDS group, it remained the original state of the nerves, with no observed nucleus and axons, the neurotoxicity grade detected by CCK\u20108 being almost 0, and it kept the largest number of Schwann cells adhered to the acellular nerve and the better morphology. Further, it showed that the selected SB\u2010SDS rats acellular nerve scaffold could promote the nerve repair of the rats by HE staining and TB staining. We could conclude that the acellular nerve matrix prepared by the SB\u2010SDS method effectively removes the cellular components in the nerve tissue and retains the main components of the extracellular matrix of the nerve tissue, whose rats decellularized nerve scaffold could promote the sciatic nerve repair better.<\/jats:p>","DOI":"10.1002\/syn.22143","type":"journal-article","created":{"date-parts":[[2019,11,9]],"date-time":"2019-11-09T18:34:58Z","timestamp":1573324498000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of SB\u2010SDS and other decellularization methods for the acellular nerve graft: Biological evaluation and nerve repair in vitro and in vivo"],"prefix":"10.1002","volume":"74","author":[{"given":"Li\u2010Wei","family":"Han","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Fourth Medical Center of PLA General Hospital Beijing People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3550-2882","authenticated-orcid":false,"given":"Gang","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Mechanism for Repair and Remodeling of Orthopaedic Diseases Dalian People\u2019s Republic of China"},{"name":"Department of Orthopaedics First Affiliated Hospital of Dalian Medical University Dalian People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei\u2010Yu","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Mechanism for Repair and Remodeling of Orthopaedic Diseases Dalian People\u2019s Republic of China"},{"name":"Department of Orthopaedics First Affiliated Hospital of Dalian Medical University Dalian People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yv\u2010Ang","family":"Chang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Mechanism for Repair and Remodeling of Orthopaedic Diseases Dalian People\u2019s Republic of China"},{"name":"Department of Orthopaedics First Affiliated Hospital of Dalian Medical University Dalian People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Mechanism for Repair and Remodeling of Orthopaedic Diseases Dalian People\u2019s Republic of China"},{"name":"Department of Orthopaedics First Affiliated Hospital of Dalian Medical University Dalian People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Tao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Fourth Medical Center of PLA General Hospital Beijing People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4735-1193","authenticated-orcid":false,"given":"Zhong\u2010Hai","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Mechanism for Repair and Remodeling of Orthopaedic Diseases Dalian People\u2019s Republic of China"},{"name":"Department of Orthopaedics First Affiliated Hospital of Dalian Medical University Dalian People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2007.02.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/4606387"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.31753"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1136(20010101)33:1<36::AID-GLIA1004>3.0.CO;2-B"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199705150-00004"},{"issue":"3","key":"e_1_2_9_7_1","first-page":"167","article-title":"Anatomy and physiology of peripheral nerve injury and repair","volume":"29","author":"Flors A. 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