{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:16:35Z","timestamp":1753870595553,"version":"3.41.2"},"reference-count":90,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T00:00:00Z","timestamp":1583452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Synapse"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dopamine D<jats:sub>3<\/jats:sub>R are widely expressed in basal ganglia where interact with D<jats:sub>1<\/jats:sub>R. D<jats:sub>3<\/jats:sub>R potentiate cAMP accumulation and GABA release stimulated by D<jats:sub>1<\/jats:sub>R in striatonigral neurons through \u201catypical\u201d signaling. During dopaminergic denervation, D<jats:sub>3<\/jats:sub>R signaling changes to a \u201ctypical\u201d in which antagonizes the effects of D<jats:sub>1<\/jats:sub>R, the mechanisms of this switching are unknown. D<jats:sub>3<\/jats:sub>nf splice variant regulates membrane anchorage and function of D<jats:sub>3<\/jats:sub>R and decreases in denervation; thus, it is possible that D<jats:sub>3<\/jats:sub>R signaling switching correlates with changes in D<jats:sub>3<\/jats:sub>nf expression and increases of membranal D<jats:sub>3<\/jats:sub>R that mask D<jats:sub>3<\/jats:sub>R atypical effects. We performed experiments in unilaterally 6\u2010hydroxydopamine lesioned rats and found a decrease in mRNA and protein of D<jats:sub>3<\/jats:sub>nf, but not of D<jats:sub>3<\/jats:sub>R in the denervated striatum. Proximity ligation assay showed that D<jats:sub>3<\/jats:sub>R\u2010D<jats:sub>3<\/jats:sub>nf interaction decreased after denervation, whereas binding revealed an increased <jats:italic>B<\/jats:italic><jats:sub>max<\/jats:sub> in D<jats:sub>3<\/jats:sub>R. The new D<jats:sub>3<\/jats:sub>R antagonized cAMP accumulation and GABA release stimulated by D<jats:sub>1<\/jats:sub>R; however, in the presence of N\u2010Ethylmaleimide (NEM), to block G<jats:sub>i<\/jats:sub> protein signaling, activation of D<jats:sub>3<\/jats:sub>R produced its atypical signaling stimulating D<jats:sub>1<\/jats:sub>R effects. Finally, we investigated if the typical and atypical effects of D<jats:sub>3<\/jats:sub>R modulating GABA release are capable of influencing motor behavior. Injections of D<jats:sub>3<\/jats:sub>R agonist into denervated nigra decreased D<jats:sub>1<\/jats:sub>R agonist\u2010induced turning behavior but potentiated it in the presence of NEM. Our data indicate the coexistence of D<jats:sub>3<\/jats:sub>R typical and atypical signaling in striatonigral neurons during denervation that correlated with changes in the ratio of expression of D<jats:sub>3<\/jats:sub>nf and D<jats:sub>3<\/jats:sub>R isoforms. The coexistence of both atypical and typical signaling during denervation influences motor behavior.<\/jats:p>","DOI":"10.1002\/syn.22152","type":"journal-article","created":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T13:13:29Z","timestamp":1582031609000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Coexistence of D<sub>3<\/sub>R typical and atypical signaling in striatonigral neurons during dopaminergic denervation. Correlation with D<sub>3<\/sub>nf expression changes"],"prefix":"10.1002","volume":"74","author":[{"given":"Baruc","family":"Campos Campos","sequence":"first","affiliation":[{"name":"Departamento de Farmacolog\u00eda Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Ciudad de M\u00e9xico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arturo","family":"\u00c1valos\u2010Fuentes","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Biof\u00edsica y Neurociencias Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Ciudad de M\u00e9xico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celia","family":"Pi\u00f1a Leyva","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Biof\u00edsica y Neurociencias Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Ciudad de M\u00e9xico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodolfo","family":"S\u00e1nchez\u2010Zavaleta","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Biof\u00edsica y Neurociencias Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Ciudad de M\u00e9xico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santiago","family":"Loya\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Ciudad de M\u00e9xico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Rangel\u2010Barajas","sequence":"additional","affiliation":[{"name":"Neurosciences Program Bloomington Indiana University Bloomington IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerardo","family":"Leyva\u2010G\u00f3mez","sequence":"additional","affiliation":[{"name":"Departamento de Farmacia Facultad de Qu\u00edmica Universidad Nacional Aut\u00f3noma de M\u00e9xico Ciudad de M\u00e9xico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hern\u00e1n","family":"Cort\u00e9s","sequence":"additional","affiliation":[{"name":"Laboratorio de Medicina Gen\u00f3mica Departamento de Gen\u00e9tica Instituto Nacional de Rehabilitaci\u00f3n Luis Guillermo Ibarra Ibarra Ciudad de M\u00e9xico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Erlij","sequence":"additional","affiliation":[{"name":"Department of Physiology SUNY Downstate Medical Center Brooklyn NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3430-9335","authenticated-orcid":false,"given":"Benjam\u00edn","family":"Flor\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Biof\u00edsica y Neurociencias Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Ciudad de M\u00e9xico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(85)90200-6"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom9090431"},{"key":"e_1_2_9_4_1","first-page":"79","article-title":"The measurement of cyclic AMP levels in biological preparations","volume":"41","author":"Alexander S. P.","year":"1995","journal-title":"Methods in Molecular Biology"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)91052-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.20372"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(88)90279-1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2014.12.008"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2013.04.010"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-3057(97)00560-1"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2013.10.036"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)91456-B"},{"key":"e_1_2_9_13_1","article-title":"CAMKII does not modulate D3 recepetor effect on [3H] Glutamate release in the rat substantia nigra","volume":"498","author":"Briones L.\u2010 S.\u2010Z.","year":"2016","journal-title":"Society for Neuroscience Abstract"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2019.03.051"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2009.07.005"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2018.11.032"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-014-1894-9"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000074976"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2016.1185413"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2012.11.032"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-006-9133-z"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(90)90148-3"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00450.2014"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)00527-C"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-23-08677.2000"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(00)00120-0"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2009.06508.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-014-8936-x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28273-5"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.107.043885"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(90)90399-T"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(98)00633-2"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(03)00298-7"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1139\/y02-081"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.68010001.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-019-1564-8"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.114.093096"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(98)00463-7"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.113.087072"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-0186(00)00047-4"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.112.083535"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(83)90131-4"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.58.4.677"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.06.017"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-17-06807.1997"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890200406"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(96)00029-2"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00529-0"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.17.8155"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1719"},{"issue":"46","key":"e_1_2_9_51_1","doi-asserted-by":"crossref","first-page":"29220","DOI":"10.1016\/S0021-9258(19)62033-8","article-title":"On the origin of mRNA encoding the truncated dopamine D3\u2010type receptor D3nf and detection of D3nf\u2010like immunoreactivity in human brain","volume":"269","author":"Liu K.","year":"1994","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.12.015"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M710349200"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-2723-1_8"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2011.07608.x"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.31.1894"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2007.07.014"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.715144"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.46.29229"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-014-1244-8"},{"key":"e_1_2_9_62_1","unstructured":"Paxinos G. &Watson C. (1997).The rat brain in stereotaxic coordinates.San Diego CA:Academic Press."},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1224-04.2004"},{"key":"e_1_2_9_64_1","first-page":"1","article-title":"Abnormalities of dopamine D3 receptor signaling in the diseased brain","volume":"9","author":"Prieto G. A.","year":"2017","journal-title":"Journal of Central Nervours System Disease"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00516.2010"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2006.06.010"},{"issue":"3","key":"e_1_2_9_67_1","first-page":"1355","article-title":"Neurochemical and functional characterization of the preferentially selective dopamine D3 agonist PD 128907","volume":"275","author":"Pugsley T. A.","year":"1995","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2015.0330"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2008.06.002"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2010.08.018"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1301163"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.20770"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-016-1306-x"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1998.00173.x"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90034-6"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom9100511"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(97)00160-7"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-24-07902.1996"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)60775-9"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-11-07109.1994"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2015.04.025"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/347146a0"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.13390"},{"issue":"3","key":"e_1_2_9_84_1","first-page":"1223","article-title":"Quantitative autoradiographic mapping of rat brain dopamine D3 binding with [(125)I]7\u2010OH\u2010PIPAT: Evidence for the presence of D3 receptors on dopaminergic and nondopaminergic cell bodies and terminals","volume":"295","author":"Stanwood G. D.","year":"2000","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.21.10178"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-20-06579.1996"},{"issue":"8","key":"e_1_2_9_87_1","doi-asserted-by":"crossref","first-page":"4315","DOI":"10.1016\/S0021-9258(19)39565-1","article-title":"KN\u201062, 1\u2010[N, O\u2010bis(5\u2010isoquinolinesulfonyl)\u2010N\u2010methyl\u2010L\u2010tyrosyl]\u20104\u2010phenylpiperazi ne, a specific inhibitor of Ca2+\/calmodulin\u2010dependent protein kinase II","volume":"265","author":"Tokumitsu H.","year":"1990","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002130100708"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1528-04.2004"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-2187-7-13"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000199983.24674.83"}],"container-title":["Synapse"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fsyn.22152","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/syn.22152","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/syn.22152","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/syn.22152","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T09:15:25Z","timestamp":1693991725000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/syn.22152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,6]]},"references-count":90,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["10.1002\/syn.22152"],"URL":"https:\/\/doi.org\/10.1002\/syn.22152","archive":["Portico"],"relation":{},"ISSN":["0887-4476","1098-2396"],"issn-type":[{"type":"print","value":"0887-4476"},{"type":"electronic","value":"1098-2396"}],"subject":[],"published":{"date-parts":[[2020,3,6]]},"assertion":[{"value":"2019-11-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22152"}}