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Continuous developments of PET tracers as biomarkers can be supported by autoradiography to validate effectiveness and accuracy of binding properties that elucidate the pathophysiology of DSAD and AD. This in vitro comparative study evaluates [\n                    <jats:sup>125<\/jats:sup>\n                    I]IPPI binding to tau and [\n                    <jats:sup>125<\/jats:sup>\n                    I]IBETA binding to A\u03b2 plaques in the frontal cortex (FCX) and temporal cortex (TCX) of postmortem human brain slices of AD (\n                    <jats:italic>n<\/jats:italic>\n                    = 5), DSAD (\n                    <jats:italic>n<\/jats:italic>\n                    = 5), and cognitively normal (CN) (\n                    <jats:italic>n<\/jats:italic>\n                    = 5) cases. With anti\u2010tau and anti\u2010A\u03b2 immunostains confirming the presence of tau and A\u03b2 plaques, [\n                    <jats:sup>125<\/jats:sup>\n                    I]IPPI and [\n                    <jats:sup>125<\/jats:sup>\n                    I]IBETA binding in autoradiographic images were significantly higher in DSAD and AD gray matter (GM) compared to CN. When comparing DSAD with AD, FCX and TCX GM binding was similar throughout DSAD and AD, except in FCX GM where there was 48% more [\n                    <jats:sup>125<\/jats:sup>\n                    I]IPPI binding in DSAD than AD. In vitro drug inhibition studies revealed that [\n                    <jats:sup>125<\/jats:sup>\n                    I]IPPI binding was significantly inhibited with increasing harmine concentrations (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 115\u00a0nM) in DSAD FCX and TCX, but KuFal194, a DYRK1A drug, minimally inhibited [\n                    <jats:sup>125<\/jats:sup>\n                    I]IPPI binding in the same cases. The GM\/white matter ratios for DSAD ([\n                    <jats:sup>125<\/jats:sup>\n                    I]IPPI = 4.1, [\n                    <jats:sup>125<\/jats:sup>\n                    I]IBETA = 2.9) and AD ([\n                    <jats:sup>125<\/jats:sup>\n                    I]IPPI = 4.2, [\n                    <jats:sup>125<\/jats:sup>\n                    I]IBETA = 2.6) were significantly greater than CN ([\n                    <jats:sup>125<\/jats:sup>\n                    I]IPPI = 1.3, [\n                    <jats:sup>125<\/jats:sup>\n                    I]IBETA = 1.2). A positive correlation between [\n                    <jats:sup>125<\/jats:sup>\n                    I]IPPI and [\n                    <jats:sup>125<\/jats:sup>\n                    I]IBETA binding suggests a synergistic relationship between tau and A\u03b2 plaque in DSAD and AD pathology. This study demonstrates that [\n                    <jats:sup>125<\/jats:sup>\n                    I]IPPI and [\n                    <jats:sup>125<\/jats:sup>\n                    I]IBETA may serve as novel radiotracers in both DSAD and AD to continue diagnostic investigations.\n                  <\/jats:p>","DOI":"10.1002\/syn.70044","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T05:31:51Z","timestamp":1773725511000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Measurement of Tau Protein and A\u03b2 Amyloid Plaques in Postmortem Human Brains of Down Syndrome and Alzheimer's Disease by Using [\n                    <sup>125<\/sup>\n                    I]IPPI and [\n                    <sup>125<\/sup>\n                    I]IBETA Autoradiography"],"prefix":"10.1002","volume":"80","author":[{"given":"Agnes P.","family":"Biju","sequence":"first","affiliation":[{"name":"Preclinical Imaging, Department of Radiological Sciences University of California\u2010Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fariha","family":"Karim","sequence":"additional","affiliation":[{"name":"Preclinical Imaging, Department of Radiological Sciences University of California\u2010Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deanna M.","family":"Schafer","sequence":"additional","affiliation":[{"name":"Preclinical Imaging, Department of Radiological Sciences University of California\u2010Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie A.","family":"Sison","sequence":"additional","affiliation":[{"name":"Preclinical Imaging, Department of Radiological Sciences University of California\u2010Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Liang","sequence":"additional","affiliation":[{"name":"Preclinical Imaging, Department of Radiological Sciences University of California\u2010Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Head","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine and Neurology University of California\u2010Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jogeshwar","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Preclinical Imaging, Department of Radiological Sciences University of California\u2010Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,16]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.22713"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401\u2010023\u201002672\u2010z"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2015.07.490"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00308809"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.22709"},{"issue":"6","key":"e_1_2_12_7_1","first-page":"546","article-title":"Biomarkers for Alzheimer's Disease. 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