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After two to three months the grafts, with or without colchicine treatment, were subjected to immunohistochemical analysis using antibodies against cholecystokinin (CCK), somatostatin (SOM), neuropeptide tyrosine (NPY), vasoactive intestinal polypeptide (VIP), peptide histidine isoleucine (PHI) and the GABA\u2010synthesizing enzyme glutamic acid decarboxylase (GAD). Cerebral cortex in situ of untreated and colchicine\u2010treated rats was always analyzed in parallel.<\/jats:p><jats:p>A dense plexus of CCK\u2010immunoreactive fibers was distributed in all parts of the transplants, and after colchicine treatment a large number of CCK\u2010positive cells was observed. These cells were markedly increased in number as compared to normal cortical tissue in colchicine\u2010pretreated rats. The amount of NPY\u2010immunoreactive cells was also markedly increased, whereas somatostatin\u2010positive cells were found in numbers similar to those seen in cortex in situ. In the grafts only a few VIP\u2010 and PHI\u2010positive fibers were seen with a few VIP\u2010positive cell bodies, but no clearly discernible PHI\u2010positive cells. A very dense plexus of GAD\u2010positive fibers with an even distribution throughout the grafts was observed. Cortex in situ exhibited a lower density of GAD\u2010immunoreactive fibers. Even after colchicine treatment the number of GAD\u2010positive cells in the grafts was low. Using double\u2010staining techniques, it was found that most of the few GAD\u2010positive cells in the grafts were also NPY\u2010positive, SOM\u2010positive or, to a minor extent, CCK\u2010positive.<\/jats:p><jats:p>The present results demonstrate that several peptides and transmitter markers are expressed in cortical grafts in oculo, but marked differences in their expression can be observed in cortical tissue that has developed in isolation. Thus, the intraocular cortex graft, alone and in combination with other brain areas, should provide a useful model in which to study factors that regulate brain development.<\/jats:p>","DOI":"10.1002\/syn.890010606","type":"journal-article","created":{"date-parts":[[2005,4,11]],"date-time":"2005-04-11T19:33:37Z","timestamp":1113248017000},"page":"539-551","source":"Crossref","is-referenced-by-count":6,"title":["Glutamic acid decarboxylase\u2010 and peptide\u2010immunoreactive neurons in cortex cerebri following development in isolation: Evidence of homotypic and disturbed patterns in intraocular grafts"],"prefix":"10.1002","volume":"1","author":[{"given":"Maria","family":"Erikdotter\u2010Nilsson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bj\u00f6urn","family":"Meister","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas","family":"H\u00f6ukfelt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Elde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Fahrenkrug","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Frey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Oertel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens F.","family":"Rehfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Terenius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Olson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"335","volume-title":"Handbook of Chemical Neuroanatomy, Vol. 4: GABA and Neuropeptides in the CNS, Part I","author":"Abrams G. 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