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Using a radioiodinated D<jats:sub>1<\/jats:sub> selective antagonist [<jats:sup>125<\/jats:sup>I]\u2010SCH 23982, we have now directly labeled and characterized the D<jats:sub>1<\/jats:sub> dopamine receptors in bovine parathyroid gland membranes.<\/jats:p><jats:p>[<jats:sup>125<\/jats:sup>I]\u2010SCH 23982 binds in a saturable manner with high affinity and low nonspecific binding to membranes prepared from bovine parathyroid glands. D<jats:sub>1<\/jats:sub> dopamine receptors are present in this preparation at a concentration of \u2248\ufe01130 fMoles\/mg protein and [<jats:sup>125<\/jats:sup>I]\u2010SCH 23982 binding increases with increasing protein concentration in a linear fashion. Determination of the K<jats:sub>d<\/jats:sub> using the association (k<jats:sub>1<\/jats:sub>) and dissociation (k<jats:sub>\u20101<\/jats:sub>) rate constants revealed good agreement with the K<jats:sub>d<\/jats:sub> determined by saturation analysis (390 pM vs. 682 pM, respectively). Inhibition of 0.3 nM [<jats:sup>125<\/jats:sup>I]\u2010SCH 23982 binding by a series of dopaminergic antagonists verified the D<jats:sub>1<\/jats:sub> nature of this binding site, exhibiting appropriate affinities and rank order of potency. The competition curves of all antagonists exhibited Hill coefficients that were not significantly different from 1. Inhibition of [<jats:sup>125<\/jats:sup>I]\u2010SCH 23982 binding by dopamine and other dopaminergic agonists revealed the presence of high and low affinity agonist binding sites. Addition of 200 \u03bcM GppNHp effected a complete conversion of high affinity dopamine binding sites to a homogeneous population of low affinity dopamine sites. The D<jats:sub>1<\/jats:sub> receptors identified in the parathyroid gland with [<jats:sup>125<\/jats:sup>I]\u2010SCH 23982 appear to be pharmacologically identical with those previously characterized in the central nervous system.<\/jats:p><jats:p>These results demonstrate that the bovine parathyroid gland, which possesses well\u2010characterized functional responses to dopaminergic stimulation, may provide a single cell\u2010type model system for future investigation of the molecular events involved in D<jats:sub>1<\/jats:sub> dopamine receptor\/response coupling and regulation.<\/jats:p>","DOI":"10.1002\/syn.890030304","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T15:34:34Z","timestamp":1104593674000},"page":"193-199","source":"Crossref","is-referenced-by-count":4,"title":["Direct demonstration of D<sub>1<\/sub> dopamine receptors in the bovine parathyroid gland using the D<sub>1<\/sub> selective antagonist [<sup>125<\/sup>I]\u2010SCH 23982"],"prefix":"10.1002","volume":"3","author":[{"suffix":"Jr.","given":"Frederick J.","family":"Monsma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David R.","family":"Sibley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-107-6-1776"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-11-03177.1986"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.10.4210"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-99-6-1582"},{"key":"e_1_2_1_6_1","first-page":"335","article-title":"Characterization of dopaminergic receptors in dispersed bovine parathyroid cells","volume":"18","author":"Brown E. 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