{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:33:42Z","timestamp":1790001222147,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,12]],"date-time":"2004-10-12T00:00:00Z","timestamp":1097539200000},"content-version":"vor","delay-in-days":4821,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Synapse"],"published-print":{"date-parts":[[1991,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Widely abused \u201cdesigner\u201d durg MDMA (3,4\u2010methylenedioxymethamphetamine) has been shown to caused marked and long\u2010lasting changes in brain serotonergic systems. The present study uses quantitative in vitro autoradiography of <jats:sup>3<\/jats:sup>H\u2010paroxetine labeled 5\u2010HT uptake sites to assess the time\u2010dependent effects of MDMA on 5\u2010HT neurons in specific neuroanatomic loci. Following treatment with MDMA (20 mg\/kg, b.i.d. for 4 days), marked decreases in 5\u2010HT uptake sites were observed in a number of brain regions known to receive projections of \u20105HT neurons. These regions included cerebral cortex, caudate nucleus, hippocampus, nucleus accumbens, olfactory tubercle, superior and inferior colliculi, geniculate nuclei, and most thalamic nuclei. In contrast, other areas such as the septal nuclei and some thalamic nuclei which also receive 5\u2010HT projections were not substantially affected by this drug. In most regions, decreases in 5\u2010HT uptake sites occurred within 24 hours of the last dose of MDMA and persisted at the 2 week time point. Some regions such as dorsal striatum exhibited a time\u2010dependent reduction with greater reductions occurring at 2 weeks rather than immediately following the MDMA treatment regimen. The density of 5\u2010HT uptake sites in other regions such as endopiriform nucleus and substantia nigra at the 2 week versus 18 hour time point indicated some degree of region\u2010specific recovery. Regions which demonstrated no significant reduction in 5\u2010HT uptake sites included the dorsal and median raphe nuclei, ventral tegmental area, central grey, interpeduncluar nucleus, locus coerulus, pontine reticular formation and cerebellum. Likewise, region containing 5\u2010HT axons of passage (e.g., indusium griseum and lateral hypothalamus) appeared to be insensitive to the neurotoxic effects of MDMA on 5\u2010HT neurons. Furthermore, the neurotoxic effects of MDMA showed specificity in that the catecholamine neurons labeled by <jats:sup>3<\/jats:sup>H\u2010mazindol were unaffected by the treatment regimen. These data indicate that the preferential degeneration of serotonergic neurons by MDMA is mediated primarily at 5\u2010HT terminal regions, whereas regions containg 5\u2010HT perikarya and axons of passage remain relatively unaffected. In addition, the observed time\u2010dependent reductions and recovery of 5\u2010HT uptake sites which were detected within 2 weeks of the treatment regimen in certain brain regions suggest region\u2010specific differences in recovery of 5\u2010HT systems from MDMA\u2010induced lesion.<\/jats:p>","DOI":"10.1002\/syn.890080403","type":"journal-article","created":{"date-parts":[[2004,12,31]],"date-time":"2004-12-31T18:57:22Z","timestamp":1104519442000},"page":"249-260","source":"Crossref","is-referenced-by-count":73,"title":["Neuroanatomic specificity and time course of alterations in rat brain serotonergic pathways induced by MDMA (3,4\u2010methylenedioxymethamphetamine): Assessment using quantitative autoradiography"],"prefix":"10.1002","volume":"8","author":[{"given":"George","family":"Battaglia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Sharkey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Kuhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Errol B.","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"Adler J. 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