{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T16:28:14Z","timestamp":1700324894602},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,10,21]],"date-time":"2002-10-21T00:00:00Z","timestamp":1035158400000},"content-version":"vor","delay-in-days":293,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Teratog Carcinog Mutagen"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ferbam, a potent dithiocarbamate fungicide is used as a protectant against a wide variety of fungal diseases in fruits, vegetables, and ornamental plants. The wide\u2010spread use of this chemical is likely to pollute the environment. Hence, it was planned to test the possible genotoxicity of Ferbam through its aneugenic potential in the in vivo mouse (<jats:italic>Mus musculus<\/jats:italic>) test system. Four different doses of Ferbam, namely, 7.5, 15.0, 30.0, 60.0 mg\/kg body weight were administered orally to mice <jats:italic>Mus musculus<\/jats:italic> suspended in gum tragacanth representing, respectively, 1\/16, 1\/8, 1\/4;, 1\/2th of the LD50 value. They were sacrificed at 6\u2010, 12\u2010, 24\u2010, and 48\u2010h intervals along with a distilled water negative control at 2 mg\/kg body weight. Colchicine treated animals were used as positive controls. Bone marrow preparations were made following the standard Hypotonic flame dry Giemsa staining technique to study the dose and time yield effect of Ferbam. The aneugenic potential was evaluated for C\u2010mitotic effects by scoring the mitotic index, c\u2010mitoses frequency, anaphase reduction, and hyper\/hypodiploidy induction. Ferbam showed a significant increase in the mitotic index and C\u2010mitoses effects and anaphase decreased at the highest doses of 30 and 60 mg\/kg at 12\u2010 and 24\u2010h intervals. Colchicine induced significant effects in all the aneugenic parameters observed at all the time intervals. There was no significant induction of either hyperdiploidy or hypodiploidy by Ferbam, unlike colchicine, indicating that the fungicide Ferbam is not aneugenic in the mouse test system. <jats:italic>Teratogenesis Carcinog. Mutagen. 22:451\u2013459, 2002.<\/jats:italic> \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/tcm.10041","type":"journal-article","created":{"date-parts":[[2002,10,23]],"date-time":"2002-10-23T12:56:35Z","timestamp":1035377795000},"page":"451-459","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of the aneugenic potential of the fungicide Ferbam in mice"],"prefix":"10.1002","volume":"22","author":[{"given":"R.","family":"Shanthi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Krishnamoorthy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,10,21]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"IPCS Reports. WHO. 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