{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:01:56Z","timestamp":1777618916073,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,9,9]],"date-time":"2005-09-09T00:00:00Z","timestamp":1126224000000},"content-version":"vor","delay-in-days":9017,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Teratog Carcinog Mutagen"],"published-print":{"date-parts":[[1981,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The YFF sperm assay, which is a quantification of the incidence of sperm with two fluorescent bodies (YFF = two fluorescent bodies), was performed to measure Y chromosomal nondisjunction. Three categories of human sub\u2010 jects were analyzed: (1) nonexposed, (2) exposed to antineoplastic agents \u2010 ie, chemo\u2010 and radiation therapy, and (3) dibromochloropropane (DBCP)\u2010exposed. The nonexposed individuals demonstrated a relatively constant mean YFF value of 1.3%, which was consistent with historical controls in this laboratory and with the results of other investigators. Further, a one\u2010way analysis of variance among the means of the control samples revealed no statistical differ\u2010 ences either between these men or within each man's samples. The individuals exposed to antineoplastic agents showed a three\u2010 to four\u2010fold increase in the incidence of YFF sperm three to six weeks after the initiation of exposure to Adriamycin\u00ae and X\u2010irradiation. The maximum percentages of YFF per 1,000 sperm for each individual in this exposed group were analyzed by Wilcoxon's distribution free rank sum test using a one\u2010sided alternative. The exposed individuals' maximum YFF percentages were statistically significantly increased when compared to the maximum YFF values of the non\u2010 exposed controls. The individuals exposed to the nematocide DBCP also exhibited a statistically significant increase in the number of sperm con\u2010 taining two Y chromosomes as determined by chi\u2010square analysis with one degree of freedom (P &lt; 0.01).<\/jats:p><jats:p>Data presented herein show statistically significant increases in the incidence of double Y chromosomes as measured by the presence of YFF sperm following exposure to Adriamycin\u00ae, X\u2010irradiation, and DBCP. It is suggested that men who have a history of antineoplastic therapy could be evaluated for evidence of Y\u2010Y nondisjunction with this method. In the event of an increased YFF sperm level, genetic counseling and amniocentesis should be made available to the spouse where pregnancy has occurred. Further, be\u2010 cause this procedure measures gametic mutation, is relatively simple, and is noninvasive, it should be considered for inclusion as part of a battery of medical tests for monitoring industrial populations.<\/jats:p>","DOI":"10.1002\/tcm.1770010208","type":"journal-article","created":{"date-parts":[[2006,2,4]],"date-time":"2006-02-04T12:17:24Z","timestamp":1139055444000},"page":"193-211","source":"Crossref","is-referenced-by-count":21,"title":["Analysis of human spermatozoa for Y chromosomal nondisjunction"],"prefix":"10.1002","volume":"1","author":[{"suffix":"Jr.","given":"R. W.","family":"Kapp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. B.","family":"Jacobson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,9,9]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"301","volume":"1","author":"DHEW Committee to Coordinate Toxicology and Related Programs by Working Group of the Subcommittee on Environmental Mutagenesis","year":"1977","journal-title":"J Environ Pathol Toxicol"},{"key":"e_1_2_1_3_2","first-page":"155","volume-title":"Work and the Health of Women","author":"Manson JM","year":"1979"},{"key":"e_1_2_1_4_2","first-page":"29","volume-title":"Les Accidents Chromosomiques de la Reproduction","author":"Bou\u00e9 A","year":"1973"},{"key":"e_1_2_1_5_2","volume-title":"Facts","author":"Anonymous, National Foundation for March of Dimes","year":"1975"},{"key":"e_1_2_1_6_2","volume-title":"Human Population Cytogenetics","author":"Smith PG","year":"1964"},{"key":"e_1_2_1_7_2","first-page":"346","volume-title":"The Genetics of the Spermatozoan","author":"Jacobs PA","year":"1972"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/00039896.1975.10666733"},{"key":"e_1_2_1_9_2","unstructured":"Department of Labor Occupational Safety and Health Administration Occupational exposure to 1 2\u2010dibromo\u20103\u2010chloropropane (DBCP). Federal Register Sept 7 1977 pp45536\u201345549."},{"key":"e_1_2_1_10_2","unstructured":"National Institute of Occupational Safety and Health Conference on Dibromochloropropane Cincinnati vols I and II Oct 20\u201321 1977."},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-3782(78)90045-2"},{"key":"e_1_2_1_12_2","unstructured":"Anonymous.National Cancer Institute\/NIEHS draft summary report on the incidence of cancer from occupational factors. Chemical Regulation Reporter Washington DC Sept 15 1978 pp1090\u20131091."},{"key":"e_1_2_1_13_2","first-page":"473","volume-title":"The Testis in Normal and Infertile Men","author":"Sherins RJ","year":"1977"},{"key":"e_1_2_1_14_2","series-title":"NIOSH Publication No. 210\u201077\u20100106","volume-title":"Sperm morphology","author":"Wyrobek AJ","year":"1981"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/274911a0"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(73)90002-X"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-0892-8_6"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1161(79)90136-5"},{"key":"e_1_2_1_19_2","first-page":"503","volume-title":"Animal Tissue Techniques","author":"Humason GL","year":"1967"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.2307\/3429138"},{"key":"e_1_2_1_21_2","volume-title":"Statistical Methods","author":"Snedecor GW","year":"1971"},{"key":"e_1_2_1_22_2","volume-title":"Nonparametric Statistical Methods","author":"Hollander M","year":"1973"},{"key":"e_1_2_1_23_2","unstructured":"JacobsonCB:Unpublished observations."},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(68)90538-7"},{"key":"e_1_2_1_25_2","first-page":"463","article-title":"Investigation of metaphase chromosomes with DNA binding fluorochromes","volume":"58","author":"Zech L","year":"1969","journal-title":"Exp Cell Res"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/226897a0"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/226961a0"},{"key":"e_1_2_1_28_2","unstructured":"SumnerAT RobinsonJA EvansHJ:Distinguishing between X Y and YY\u2010bearing human spermatoza."},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0480009"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/262493a0"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1159\/000130746"},{"key":"e_1_2_1_32_2","first-page":"144","volume-title":"The Genetics of the Spermatozoan","author":"Evans HJ","year":"1972"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00393995"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0220177"}],"container-title":["Teratogenesis, Carcinogenesis, and Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Ftcm.1770010208","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tcm.1770010208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,4]],"date-time":"2023-11-04T00:35:38Z","timestamp":1699058138000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tcm.1770010208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,1]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1981,1]]}},"alternative-id":["10.1002\/tcm.1770010208"],"URL":"https:\/\/doi.org\/10.1002\/tcm.1770010208","archive":["Portico"],"relation":{},"ISSN":["0270-3211","1520-6866"],"issn-type":[{"value":"0270-3211","type":"print"},{"value":"1520-6866","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,1]]}}}