{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T18:28:16Z","timestamp":1764700096482},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,9,9]],"date-time":"2005-09-09T00:00:00Z","timestamp":1126224000000},"content-version":"vor","delay-in-days":4269,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Teratog Carcinog Mutagen"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to define the long\u2010term stability of metabolizing enzymes in activating preparations for short\u2010term genotoxicity bioassays under various storage conditions. Expressions of cytochrome P450 content, NADPH\u2010cytochrome (P450) c\u2010re\u2010ductase activity, and of the several monooxygenases, such as aminopyrine N\u2010demethylase (class IIIA P450), p\u2010nitroanisole O\u2010demethylase (mixed), dinemorphan N\u2010demethylase (HB1), ethoxyresorufin O\u2010deethylase (IA1), ethoxycoumarin O\u2010deethylase (mixed), and pentoxyresorufin O\u2010dealkylase (IIB1), were examined in S9 fractions derived from Na\u2010phenobarbital (PB) plus \u03b2\u2010naphthoflavone (\u03b2\u2010NF) induced male and female mice, stored at \u221280\u00b0C, or lyophilized and stored at \u221220\u00b0C. Lipid peroxidation was also determined. Cytochrome P450 and the associated activities were decreased by 30\u201382% within 9 months of storage. The pattern and degree of relative stabilities were different for the various isoforms. The IA 1\u2010like activity, for example, was much more stable (\u02dc49%loss) than IIB 1\u2010like activities (up to 82% loss). In general, lyophilized enzymes were less stable than directly frozen preparations. In addition, immediately after freeze\u2010drying (lyophilization), a marked decrease in activity of up to 35% was observed. On the contrary, demethylation of aminopyrine and p\u2010nitroanisole remains almost constant over 6 months storage at \u2212196\u00b0C. The results obtained indicate that either fresh, daily made S9 fractions or, alternatively, fractions stored in liquid nitrogen (up to 6 months) are recommended for mutagenesis studies. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/tcm.1770140103","type":"journal-article","created":{"date-parts":[[2006,2,4]],"date-time":"2006-02-04T01:27:10Z","timestamp":1139016430000},"page":"13-22","source":"Crossref","is-referenced-by-count":30,"title":["Stability of microsomal monooxygenases in murine liver S9 fractions derived from phenobarbital and \u03b2\u2010naphthoflavone induced animals under various long\u2010term conditions of storage"],"prefix":"10.1002","volume":"14","author":[{"given":"Carlo","family":"Bauer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Corsi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moreno","family":"Paolini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,9,9]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"301","article-title":"Expression and inducibility of drug metabolizing enzymes in novel murine liver epithelial cell lines and their ability to activate precarcinogens","volume":"51","author":"Paolini M","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/12.5.759"},{"key":"e_1_2_1_4_2","first-page":"225","volume-title":"Bambury Reports 2","author":"Dent GC","year":"1979"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/em.2860030208"},{"key":"e_1_2_1_6_2","first-page":"1783","article-title":"Studies on microsomal metabolizing systems for mutagenesis tests. Stability of fortified fractions","volume":"56","author":"Bauer C","year":"1981","journal-title":"Boll Soc It Biol Sper"},{"key":"e_1_2_1_7_2","first-page":"397","article-title":"Stability of drug metabolizing activities in lyophilized liver and lung microsomal preparations","volume":"2","author":"Patel JM","year":"1983","journal-title":"Mutagenesis"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/2.5.397"},{"key":"e_1_2_1_9_2","first-page":"413","volume-title":"Comparative Genetic Toxicology","author":"Hubbard SA","year":"1987"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/8.9.1179"},{"key":"e_1_2_1_11_2","first-page":"556","volume-title":"Fundamentals of Drug Metabolism and Drug Disposition","author":"Mazel P","year":"1971"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0550416"},{"key":"e_1_2_1_13_2","first-page":"556","volume-title":"Fundamentals of Drug Metabolism and Drug Disposition","author":"Zannoni VG","year":"1971"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(85)90138-9"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01963588"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90245-2"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90114-7"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(67)10094-3"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(78)52032-6"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(79)80033-0"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_22_2","first-page":"340","volume-title":"Techniques in protein chemistry","author":"Bailey JL","year":"1967"},{"key":"e_1_2_1_23_2","first-page":"80","volume-title":"Statistics for Experiments","author":"Box GEP","year":"1978"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(83)90141-0"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0160-5402(80)90001-7"},{"key":"e_1_2_1_26_2","first-page":"7","article-title":"Characterization of S9 fraction used in the third UKEMS collaborative trial","volume":"5","author":"Hubbard SA","year":"1990","journal-title":"Mutagenesis"}],"container-title":["Teratogenesis, Carcinogenesis, and Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Ftcm.1770140103","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tcm.1770140103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T05:39:30Z","timestamp":1698212370000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tcm.1770140103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1002\/tcm.1770140103"],"URL":"https:\/\/doi.org\/10.1002\/tcm.1770140103","archive":["Portico"],"relation":{},"ISSN":["0270-3211","1520-6866"],"issn-type":[{"value":"0270-3211","type":"print"},{"value":"1520-6866","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1]]}}}