{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T22:28:12Z","timestamp":1785709692933,"version":"3.56.0"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,5,5]],"date-time":"2005-05-05T00:00:00Z","timestamp":1115251200000},"content-version":"vor","delay-in-days":13518,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Teratology"],"published-print":{"date-parts":[[1968,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The teratogenic effects of N\u2010nitroso\u2010N\u2010methylurea on the rat embryo were investigated. Skeletal malformations were produced in rat embryos by a dose of 20 mg\/kg of maternal body weight on day 13 of pregnancy. The structurally malformed fetuses had multiple malformations including: clubfeet, syndactyly, cleft palate, micrognathia, crooked tail, and hydrocephalus. Drug administered on day 8 or 10 at dosages of 5\u201320 mg\/kg gave no malformations at term but tended to increase fetal mortality. A dose of 20 mg\/kg on day 13, however, resulted in a high incidence of congenital malformations but did not increase the resorption rate. To correlate the structural malformations with biochemical changes, zone electrophoretic separation of several enzyme systems was carried out in starch and polyacrylamide gels. Variations from the normal isozyme patterns of lactate dehydrogenase, glucose\u20106\u2010phosphate dehydrogenase, and nonspecific esterases were found in abnormally developing embryos and organs.<\/jats:p>","DOI":"10.1002\/tera.1420010207","type":"journal-article","created":{"date-parts":[[2005,6,10]],"date-time":"2005-06-10T23:29:16Z","timestamp":1118446156000},"page":"179-191","source":"Crossref","is-referenced-by-count":15,"title":["Effects of N\u2010nitroso\u2010N\u2010methylurea on enzymatic ontogeny associated with teratogenesis"],"prefix":"10.1002","volume":"1","author":[{"given":"E. Marshall","family":"Johnson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Lambert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,5,5]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600540127"},{"key":"e_1_2_1_3_1","first-page":"52","article-title":"The protection of Escherichia coli against inhibition by 1\u2010methyl\u20103\u2010nitro\u20101\u2010nitrosoguanidine","volume":"11","author":"Greenberg J.","year":"1961","journal-title":"Antibiot. Chemother."},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(58)90509-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01163a515"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-118-29742"},{"key":"e_1_2_1_7_1","unstructured":"Johnson E. M.1968Ele\u0107trophoretic analysis of embryonic tissues. (Selected papers from the second and third Workshops in Teratology; in press.)"},{"key":"e_1_2_1_8_1","first-page":"271","article-title":"Enzymic differentiation of rat yolk\u2010sac placenta as affected by a teratogenic agent","volume":"16","author":"Johnson E. M.","year":"1966","journal-title":"J. Embryol. Exp. Morph."},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","unstructured":"Johnson E. M. andR.Spinuzzi1967Differentiation of alkaline phosphatase and glucose\u20106\u2010phosphate dehydrogenase in the rats' yolk\u2010sac. J. Embryol. Exp. Morph. (In press.)","DOI":"10.1242\/dev.19.2.137"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.140.3573.1329"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(62)90019-2"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00632394"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1093\/jn\/56.3.349","article-title":"Multiple congenital abnormalities resulting from transitory deficiency of pteroylglutamic acid during gestation in the rat","volume":"56","author":"Nelson M. M.","year":"1955","journal-title":"J. Nutr."},{"key":"e_1_2_1_14_1","first-page":"802","article-title":"Untersuchungen uber die mutagene Wirkung verschiedener Nitrosamin\u2014und Nitrosamid\u2014Verbindugen","volume":"14","author":"Pasternak Von L.","year":"1964","journal-title":"Arzneimittelforschung"},{"key":"e_1_2_1_15_1","first-page":"246","article-title":"Der Einfluss von Diathylnitrosamin auf Glukosephosphorylierende Fermente der Mauseleber","volume":"14","author":"Sydow G.","year":"1965","journal-title":"Acta Biol. Med. Ger."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00524146"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1001\/archpedi.1942.02010110092009"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/ct9191501093"},{"key":"e_1_2_1_19_1","first-page":"251","volume-title":"Teratology: Principles and Techniques","author":"Wilson J. G.","year":"1965"}],"container-title":["Teratology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Ftera.1420010207","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tera.1420010207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T09:20:48Z","timestamp":1699780848000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tera.1420010207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,5]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1968,5]]}},"alternative-id":["10.1002\/tera.1420010207"],"URL":"https:\/\/doi.org\/10.1002\/tera.1420010207","archive":["Portico"],"relation":{},"ISSN":["0040-3709","1096-9926"],"issn-type":[{"value":"0040-3709","type":"print"},{"value":"1096-9926","type":"electronic"}],"subject":[],"published":{"date-parts":[[1968,5]]}}}