{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T21:39:22Z","timestamp":1766266762004},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,5,9]],"date-time":"2005-05-09T00:00:00Z","timestamp":1115596800000},"content-version":"vor","delay-in-days":11908,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Teratology"],"published-print":{"date-parts":[[1972,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pregnant Swiss\u2010Webster mice received 20 mg\/kg cyclophosphamide ip on day 11 of gestation and 100\u2013125 \u03bcCi\/kg <jats:sup>14<\/jats:sup>C\u2010labeled thymidine, orotic acid, uridine, or leucine at various times afterwards. DNA synthesis in embryos, as measured by <jats:sup>14<\/jats:sup>C\u2010thymidine incorporation, was significantly reduced at 12 h but not 3, 6, 24, 48, or 72 h after treatment, and embryonic DNA was reduced at 12, 24, 48, and 72 h. <jats:sup>14<\/jats:sup>C\u2010thymidine incorporation and DNA content were also reduced in placentas and maternal livers after a delay of at least 6 h. Embryonic protein synthesis, as measured by 14C\u2010leucine incorporation, was significantly reduced at 72 and 96 h after treatment. Embryonic RNA synthesis or content was not significantly affected. It was concluded that a teratogenic dose of cyclophosphamide quantitatively affects the synthesis of DNA and protein in the mouse embryo. The temporal aspects of the changes indicated that the effects on DNA synthesis may be the cause of induced developmental defects but effects on protein synthesis appear to be the result rather than the cause of the defects.<\/jats:p>","DOI":"10.1002\/tera.1420060203","type":"journal-article","created":{"date-parts":[[2005,6,10]],"date-time":"2005-06-10T22:33:23Z","timestamp":1118442803000},"page":"129-137","source":"Crossref","is-referenced-by-count":19,"title":["The <i>in vivo<\/i> biosynthesis of DNA, RNA, and proteins by mouse embryos after a teratogenic dose of cyclophosphamide"],"prefix":"10.1002","volume":"6","author":[{"given":"Robert D.","family":"Short","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. 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