{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T13:10:24Z","timestamp":1699794624507},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,5,18]],"date-time":"2005-05-18T00:00:00Z","timestamp":1116374400000},"content-version":"vor","delay-in-days":9178,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Teratology"],"published-print":{"date-parts":[[1980,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Midpalate was analyzed for the presence of nonmuscle contractile systems. The results indicate that increased amounts of actin and myosin are present in cells of regions 2 and 3. A localization of the contractile proteins in cellular projections directed against smooth muscle myosin and against skeletal muscle actin. Specificity of the immunofluorescence reactions was ascertained by immunoabsorption studies using purified myosin and actin. Electron microscopic observations of the mesenchymal cells in region 2 revealed 70\u00c5 microfilaments along the cell periphery and packed in fliopodia\u2010like projections which course between the cells. These cells, which surround a small ossification center, show no orientation, but extend up to the cranial base perichondrium and down into the shelf between the tongue side epithelium and the ossification center. The cells and projections are attached to each other by adherens and tight\u2010like junctions, forming a putative cohesive contractile network. Putative contractile cells in region 3 are strikingly aligned perpendicular to the oral epithelium and extend one\u2010third of the distance into the shelf. Projections from region 3 cells are contiguous with basement membrane material of the oral epithelium. Axonal bundles and single axons were commonly observed coursing through regions 2 and 3, often seen in close association with the mesenchymal cells. Both clear and dense\u2010core vesicles were found in the axons and cells of these regions. The possible role of these putative nonmuscle contractile cells in palate morphogenesis is discussed.<\/jats:p>","DOI":"10.1002\/tera.1420210212","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T14:33:24Z","timestamp":1118500404000},"page":"209-223","source":"Crossref","is-referenced-by-count":5,"title":["Palate morphogenesis. I. Immunological and ultrastructural analyses of mouse palate"],"prefix":"10.1002","volume":"21","author":[{"given":"Evelyn Meyer","family":"Kuhn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce S.","family":"Babiarz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James L.","family":"Lessard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernest F.","family":"Zimmerman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091870304"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(75)90261-4"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420200210"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.46.070177.004053"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(75)90021-6"},{"key":"e_1_2_1_7_1","first-page":"555","article-title":"Palatal elevation in the Wistar rat fetus","volume":"125","author":"Ferguson M. 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