{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:34:56Z","timestamp":1760204096965},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,5,19]],"date-time":"2005-05-19T00:00:00Z","timestamp":1116460800000},"content-version":"vor","delay-in-days":8205,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Teratology"],"published-print":{"date-parts":[[1982,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of this study was to see what, if any, cellular changes occurred in the mouse embryo following a single injection of ethanol, a known teratogen in humans and animals, on day 9 of gestation. No changes were seen until 6 hours after injection, when many degenerating cells and necrotic fragments were seen in the neuroepithelium of the neural groove and of the neural tube. In addition, large clear vacuoles were seen in the cytoplasm of many cells and the pseudopodia at the luminal side of the neural groove appeared swollen. The cytoplasm of the latter also contained vacuoles. When tritiated thymidine was injected 5 hours after ethanol and 1 hour before sacrifice, many degenerating cells were labelled. In addition, many cells with labelled nuclei had abnormal vacuoles in the cytoplasm. Hence, it is likely that the toxicity of ethanol is exerted primarily on some component of the cytoplasm and not on DNA synthesis. Twelve hours after ethanol, the cytoplasmic vacuoles and swollen pseudopodia had disappeared, but dying cells were still evident. By 24 hours, the necrotic debris had been completely phagocytosed by healthy neuroepithelial cells. By 50 hours, the neuroepithelium had been cleared of cell debris, although many ethanol\u2010treated embryos had open defects of the cranial neural tube. Treatment of pregnant mice with single doses of acetaldehyde, also an established teratogen in animals, did not produce any cellular changes. However, a single dose of acetaldehyde is rapidly metabolized by the mother, and would not be comparable to the small but continuous blood levels that a dose of ethanol would produce. Hence, we could not conclude with certainty that the cytotoxic effects of ethanol were exerted directly.<\/jats:p>","DOI":"10.1002\/tera.1420260305","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T14:44:35Z","timestamp":1118501075000},"page":"247-254","source":"Crossref","is-referenced-by-count":57,"title":["Ethanol teratogenicity in mice: A light microscopic study"],"prefix":"10.1002","volume":"26","author":[{"given":"John","family":"Bannigan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Burke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,19]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"668","article-title":"Ethanol\u2010induced prenatal growth deficiency: changes in fetal body composition","volume":"211","author":"Abel E. L.","year":"1979","journal-title":"J. Pharmacol. Exper. Ther."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.573922"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420150303"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420220110"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197805112981906"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1980.tb05627.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091890407"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(71)90746-0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420120102"},{"key":"e_1_2_1_11_1","first-page":"137A","article-title":"A formaldehyde\u2013glutaraldehyde fixative of high osmolarity for use in electron microscopy","volume":"27","author":"Karnovsky M. J.","year":"1965","journal-title":"J. Cell. Biol."},{"key":"e_1_2_1_12_1","volume-title":"Biochemistry of Alcohol and Alcoholism","author":"Kricka L. J.","year":"1979"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(16)33334-8"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420160104"},{"key":"e_1_2_1_15_1","first-page":"261","article-title":"The role of extracellular material in chick neurulation. 1. Effects of concanavilin A","volume":"198","author":"Lee H.","year":"1976","journal-title":"J. Exp. Morph."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-7125(16)32242-8"},{"key":"e_1_2_1_17_1","first-page":"65","article-title":"The teratogenic effects of acetaldehyde: implications for the study of the fetal alcohol syndrome","volume":"128","author":"O'Shea K. S.","year":"1979","journal-title":"J. Anat. (Lond.)"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420190209"},{"key":"e_1_2_1_19_1","first-page":"659","volume-title":"2nd International Symposium on Alcohol and Aldehyde Metabolising Systems","author":"Randall C. L.","year":"1976"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(77)90046-2"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091910307"},{"key":"e_1_2_1_22_1","first-page":"167","article-title":"The influence of aethyl\u2010alcohol on the developing chick embryo. 11","volume":"5","author":"Sandor S.","year":"1968","journal-title":"Rev. Roum. Embryol. Cytol. Serie Embryol."},{"key":"e_1_2_1_23_1","first-page":"105","article-title":"The action of aethanol on the praenatal development of albino rats","volume":"8","author":"Sandor S.","year":"1971","journal-title":"Rev. Roum. Embryol. Cytol. Serie Embryol."},{"key":"e_1_2_1_24_1","first-page":"171","article-title":"The fetal alcohol syndrome: symptoms and pathogenesis","volume":"19","author":"Veghelyi P. V.","year":"1978","journal-title":"Acta Paediatr. Acad. Sci. Hung."},{"key":"e_1_2_1_25_1","first-page":"180","article-title":"Placental transfer of ethanol and its elimination at term","volume":"40","author":"Waltman R.","year":"1972","journal-title":"Obstet. Gynecol."},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001460204"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420210110"}],"container-title":["Teratology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Ftera.1420260305","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tera.1420260305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T05:01:37Z","timestamp":1699765297000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tera.1420260305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,12]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1982,12]]}},"alternative-id":["10.1002\/tera.1420260305"],"URL":"https:\/\/doi.org\/10.1002\/tera.1420260305","archive":["Portico"],"relation":{},"ISSN":["0040-3709","1096-9926"],"issn-type":[{"value":"0040-3709","type":"print"},{"value":"1096-9926","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,12]]}}}