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Cell and tissue damage within cornea and mesenchyme were assessed. Corneas from the treated groups were undulating and uneven owing to abnormal lens placement and corneal cell injury. Extensive extracellular spacing, few cell\u2010to\u2010cell contacts, patchy glycocalyces, and abnormal surface morphology characterized the corneal cells of treated tissues. Mesenchyme of severely malformed eyes typically filled the eye field in regions normally occupied by retina and lens. As a tissue, mesenchyme lacked organization and those surface features normally present in component cells were altered. Mesenchymal cells were packed so closely that extracellular spaces were nearly obliterated. Microvilli, filopodia, and lamellipodia, cell processes observed in normal mesenchyme, were altered in both number and structure. The cytoplasm of mesenchymal cells was condensed and decreased in volume. These data suggested that (1) in this trypan blue ocular model, virtually all major tissue types were morphologically altered; (2) because healthy mesenchyme and cornea are required for normal development of lens, optic vesicle and ocular adnexa it follows that abnormal cornea and mesenchyme also will compromise these tissues; and (3) since necrotic reminants were not present, this trypan blue model probably causes developmental arrest. The most likely teratogenic pathway is presented with emphasis on the cell surface as a probable site of dye action.<\/jats:p>","DOI":"10.1002\/tera.1420290113","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T14:48:43Z","timestamp":1118501323000},"page":"105-116","source":"Crossref","is-referenced-by-count":0,"title":["Ultrastructure of trypan blue\u2010induced ocular defects II. Cornea and mesenchyme"],"prefix":"10.1002","volume":"29","author":[{"given":"Karmen L.","family":"Schmidt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Milner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela J.","family":"Hilburn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waldemar A.","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,31]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"93","article-title":"Contributions to the study of bisazo dye induced eye anomalies in rats","volume":"23","author":"Amels D.","year":"1977","journal-title":"Rev. Roum. Morphol. Embryol. 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