{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:00:20Z","timestamp":1762160420632},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T00:00:00Z","timestamp":1117152000000},"content-version":"vor","delay-in-days":6752,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Teratology"],"published-print":{"date-parts":[[1986,12]]},"abstract":"Abstract<\/jats:title>Male mice heterozygous for the dominant polydactyly gene Pdn<\/jats:italic> (Polydactyly Nagoya) were crossed with normal or heterozygous females of the same strain. Pregnant females were treated with 5 mg\/kg of cytosine arabinoside (Ara\u2010C) on day 12 of gestation. The offspring were removed on day 18 of gestation and examined for external malformations, and the fore\u2010 and hindlimbs were examined by means of bone\u2010 and cartilage\u2010stained cleared specimens.<\/jats:p>In +\/+ x Pdn<\/jats:italic>\/+ matings, Pdn<\/jats:italic>\/+ fetuses, bearing preaxial polydactyly of the distal phalangeal type in the hindlimb and deformity of the 1st digit in the forelimb, were obtained in about 50% of the nontreated group. In treated fetuses, however, the incidence of polydactyly and deformity of the 1st digit decreased to 1.4 and 10.1%, respectively. Nontreated Pdn<\/jats:italic>\/Pdn<\/jats:italic> fetuses exhibited preaxial polydactyly of the duplicated or triplicated metacarpal\/metatarsal type both in the fore\u2010 and hindlimbs. In the treated Pdn<\/jats:italic>\/Pdn<\/jats:italic> fetuses, the number of preaxial extra digits decreased in both limbs. Some hindlimbs of the treated Pdn<\/jats:italic>\/Pdn<\/jats:italic> fetuses exhibited five metatarsals, normally.<\/jats:p>In the vitally stained specimens at 6 and 24 hours after injection of Ara\u2010C, preaxial marginal necrotic zones (fMI) were observed in almost all of the treated embryos from +\/+ \u00d7 Pdn<\/jats:italic>\/ + matings. However, approximately half of the embyros did not exhibit fMI in the nontreated control group at the same stage. Those embryos deficient in fMI were regarded as Pdn<\/jats:italic>\/ +.<\/jats:p>These findings indicated that a subteratogenic dose of Ara\u2010C prevented the genetic expression of polydactyly in almost all Pdn<\/jats:italic>\/+ and some cases of Pdn<\/jats:italic>\/Pdn<\/jats:italic> mice. In addition, the fMI had an important role in the morphogenesis of preaxial polydactyly of distal phalangeal type in Pdn<\/jats:italic>\/+ mice.<\/jats:p>","DOI":"10.1002\/tera.1420340308","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T15:00:33Z","timestamp":1118502033000},"page":"283-289","source":"Crossref","is-referenced-by-count":15,"title":["Prevention of polydactyly manifestation in Polydactyly Nagoya (Pdn<\/i>) mice by administration of cytosine arabinoside during pregnancy"],"prefix":"10.1002","volume":"34","author":[{"given":"Ichiro","family":"Naruse","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiro","family":"Kameyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(57)90436-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(68)90058-0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/3.2.223"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401640313"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.152.3730.1766"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1093\/oxfordjournals.jhered.a108366","article-title":"Prevention of otolith defect in pastel mink by manganese supplementation","volume":"64","author":"Erway L. 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