{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:51:06Z","timestamp":1790787066044,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T00:00:00Z","timestamp":1173916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The MolDarT is a novel short\u2010term assay for testing mechanism\u2010based molecular effects in developing zebrafish embryos. The objective of this study was to evaluate the inducibility of vitellogenin1 mRNA (<jats:italic>Vtg1<\/jats:italic>) by the estrogenically active compounds 17\u03b2\u2010Estradiol (E2), 17\u03b1\u2010Ethinylestradiol (EE2), Nonylphenol (NP), Bisphenol A (BPA), Cyproconazol, and the suspected xeno\u2010estrogen Atrazin in the MolDarT. Freshly fertilized zebrafish eggs were exposed semistatically for 120 h. Using reverse transcription real\u2010time PCR, the relative abundance of <jats:italic>Vtg1<\/jats:italic> was measured. For EE2 a dose\u2010response relationship was established with EC50 = 60.7 ng\/L (205 pM). Induction of <jats:italic>Vtg<\/jats:italic>1 was significant at concentrations of 84 pM EE2 (25 ng EE2\/L) and above, 10 nM E2 (2.7 \u03bcg E2\/L), 100 nM E2 (27 \u03bcg E2\/L), 10 \u03bcM BPA (2280 \u03bcg BPA\/L), and 15 \u03bcM BPA (3420 \u03bcg BPA\/L). At NP concentrations of 0.75 \u03bcM (165 \u03bcg NP\/L) and 1.5 \u03bcM (330 \u03bcg NP\/L) <jats:italic>Vtg1<\/jats:italic> was significantly down\u2010regulated. Both atrazine and cyproconazol showed no effect on relative <jats:italic>Vtg1<\/jats:italic> abundance. With this study we further characterize the MolDarT assay and show its applicability for effect screening of compounds. \u00a9 2007 Wiley Periodicals, Inc. 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