{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T01:36:22Z","timestamp":1787621782352,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,1,23]],"date-time":"2008-01-23T00:00:00Z","timestamp":1201046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Molecular effect detection is a useful approach for ecotoxicological screening of chemicals. We show here the application of the molecular DarT (MolDarT), where the expression of selected target genes is detected in short\u2010term (120 h) exposed developing zebrafish (<jats:italic>Danio rerio<\/jats:italic>), thus allowing subacute multi\u2010effect compound screening. The genes metallothionein 2 (<jats:italic>mt<\/jats:italic><jats:italic>2<\/jats:italic>), cytochrome P450 1A1 (<jats:italic>cyp<\/jats:italic><jats:italic>1a<\/jats:italic><jats:italic>1<\/jats:italic>), and recombination activation gene 1 (<jats:italic>rag<\/jats:italic><jats:italic>1<\/jats:italic>) are used as endpoints that describe detoxification\/metal toxicity (<jats:italic>mt<\/jats:italic><jats:italic>2<\/jats:italic>), detoxification\/PAH toxicity (<jats:italic>cyp<\/jats:italic><jats:italic>1a<\/jats:italic><jats:italic>1<\/jats:italic>), and acquired immune system disruption (<jats:italic>rag<\/jats:italic><jats:italic>1<\/jats:italic>). Each gene's developmental expression was studied in unexposed zebrafish during 4 to 120 h past fertilization (hpf), and all three genes were found to be expressed at 120 hpf. Furthermore, <jats:italic>mt<\/jats:italic><jats:italic>2<\/jats:italic> transcripts were present at high levels at 4 hpf, indicating a maternal transfer. For positive toxicity controls, freshly fertilized zebrafish eggs were exposed for 120 hpf to ZnSO<jats:sub>4<\/jats:sub>, 1,5\u2010dimethylnaphthalene (DMN) and CdCl<jats:sub>2<\/jats:sub>. Exposure to 100 and 200 \u03bcM ZnSO<jats:sub>4<\/jats:sub> significantly induced <jats:italic>mt<\/jats:italic><jats:italic>2<\/jats:italic>; 10 \u03bcM DMN and 20 \u03bcM DMN resulted in significantly increased <jats:italic>cyp<\/jats:italic><jats:italic>1a<\/jats:italic><jats:italic>1<\/jats:italic> abundance; and 5 and 10 \u03bcM CdCl<jats:sub>2<\/jats:sub> significantly reduced <jats:italic>rag<\/jats:italic><jats:italic>1<\/jats:italic> expression levels. Furthermore, we analysed these target genes for their expression in zebrafish eggs from a previous exposure study. The eggs were exposed for 120 hpf to the environmental pollutants estradiol (E2), ethinylestradiol (EE2), nonylphenol (NP), atrazine, cyproconazol, and bisphenol A (BPA) and found differential expression of the three genes. Exposure to the (xeno\u2010)estrogenic compound NP (0.75 \u03bcM) significantly lowered <jats:italic>mt<\/jats:italic><jats:italic>2<\/jats:italic> expression. This study shows the potential of short\u2010term <jats:italic>in vivo<\/jats:italic> multi\u2010effect screenings within one single subacute exposure using the MolDarT. \u00a9 2008 Wiley Periodicals, Inc. Environ Toxicol, 2008.<\/jats:p>","DOI":"10.1002\/tox.20305","type":"journal-article","created":{"date-parts":[[2008,1,23]],"date-time":"2008-01-23T19:47:07Z","timestamp":1201117627000},"page":"59-67","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Molecular multi\u2010effect screening of environmental pollutants using the MolDarT"],"prefix":"10.1002","volume":"23","author":[{"given":"Anja","family":"Liedtke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Muncke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"R\u00fcfenacht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rik I. 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