{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T03:14:03Z","timestamp":1784776443812,"version":"3.55.0"},"reference-count":67,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,4,4]],"date-time":"2012-04-04T00:00:00Z","timestamp":1333497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:p>This study was aimed to investigate the reproductive health in adult male rats exposed to di\u2010<jats:italic>n<\/jats:italic>\u2010butyl phthalate (DBP) during embryonic development. Pregnant rats were injected with DBP and F1 male rats were weaned and on postnatal day 100, used for mating with normal cycling females to assess reproductive performance. After completion of cohabitation period, rats were analyzed for other reproductive end points. Transplacental exposure to DBP significantly decreased fertility in adult male rats. Prenatal exposure to DBP significantly decreased sperm density, number of motile sperms, viable sperms, and hypoosmotic swelling tail coiled sperms with an increase in morphological abnormalities in sperms. Testicular steroidogenic enzyme activity levels and serum testosterone levels were significantly decreased in rats exposed to DBP during embryonic development. In conclusion, transplacental exposure to DBP impairs male reproductive performance by decreasing steroidogenesis and spermatogenesis. \u00a9 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 534\u2013544, 2014.<\/jats:p>","DOI":"10.1002\/tox.21779","type":"journal-article","created":{"date-parts":[[2012,4,4]],"date-time":"2012-04-04T16:27:44Z","timestamp":1333556864000},"page":"534-544","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Prenatal di\u2010<i>n<\/i>\u2010butyl phthalate exposure alters reproductive functions at adulthood in male rats"],"prefix":"10.1002","volume":"29","author":[{"given":"Nelli","family":"Giribabu","sequence":"first","affiliation":[{"name":"Department of Zoology, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India &gt;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sri Bhashyam","family":"Sainath","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamanji","family":"Sreenivasula Reddy","sequence":"additional","affiliation":[{"name":"Department of Zoology, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India &gt;"},{"name":"Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,4,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(98)00065-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0108"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfg087"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(97)81446-X"},{"key":"e_1_2_6_6_1","first-page":"10","volume-title":"Laboratory Manual for the Examination of Human Semen and Semen\u2010Cervical Mucus Interaction","author":"Belsey MA","year":"1980"},{"key":"e_1_2_6_7_1","first-page":"447","volume-title":"Methods of Enzymatic Analysis","author":"Bergmayer HU","year":"1974"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2010.04.005"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.93101378"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfi011"},{"key":"e_1_2_6_11_1","first-page":"257","article-title":"CPCSEA guidelines for laboratory animal facility","volume":"35","author":"CPCSEA (Committee for the Purpose of Control and Supervision on Experiments on Animals)","year":"2003","journal-title":"Indian J Pharmacol"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/01926230600642625"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520292609115636"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02790.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4274(93)90104-6"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(94)90002-7"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(99)00192-7"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0890-6238(99)00066-0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00025"},{"key":"e_1_2_6_20_1","first-page":"251","article-title":"Function of seminal vesicles and their role on male fertility","volume":"3","author":"Gonzales GF","year":"2001","journal-title":"Asian J Androl"},{"key":"e_1_2_6_21_1","first-page":"115","article-title":"Homosexual behavior in prenatally stressed male rats after castration and oestrogen treatment in adulthood","volume":"76","author":"Gotz G","year":"1980","journal-title":"Endokrinologie"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1191\/074823399678846664","article-title":"Administration of potentially antiandrogenic pesticides (procymidone, linuron, iprodione, chlozolinate, p,p\u2032\u2010DDE, and ketoconazole) and toxic substances (dibutyl\u2010 and diethylhexyl phthalate, PCB 169, and ethane dimethane sulphonate) during sexual differentiation produces diverse profiles of reproductive malformations in the male rat","volume":"15","author":"Gray LE","year":"1999","journal-title":"Toxicol Ind Health"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/58.2.350"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/7.3.248"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0890114"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ede.0000235996.89953.d7"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/del428"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijheh.2007.07.011"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.3109\/01485019208987714"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfn140"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ede.0000164555.19041.01"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2004.10.009"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(98)00037-0"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287390490514859"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.037184"},{"key":"e_1_2_6_36_1","first-page":"553","volume-title":"Reproductive and Developmental Toxicology","author":"Klinefelter GR","year":"1998"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(02)00414-9"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2005.00607.x"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfh169"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0830611"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0890-6238(92)90034-Q"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.039404"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2005.00642.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/10937400903094091"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2009.01005.x"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.9366"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.8075"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0370179"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2007.09.004"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2008.0268"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1080\/10915810701352721"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/43.1.47"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1999.8643"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/55.1.143"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0890-6238(01)00201-5"},{"key":"e_1_2_6_57_1","first-page":"769","article-title":"Effect on histological and sperm kinetics in DBP exposed Wistar rats","volume":"29","author":"Nair N","year":"2008","journal-title":"J Environ Biol"},{"key":"e_1_2_6_58_1","first-page":"125","volume-title":"Guide for the Care and Use of Laboratory Animals. Institute of Laboratory Animal Resources (ILAR), Commission on Life Sciences","author":"NRC (National Research Council)","year":"1996"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/58.2.339"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1990.tb02056.x"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287391003751760"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00128-004-0374-4"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402150210"},{"key":"e_1_2_6_64_1","first-page":"179","article-title":"Effects of dibutyl phthalate on sertoli cells of rat testis","volume":"39","author":"Wang YB","year":"2005","journal-title":"Zhonghua Yu Fang Yi Xue Za Zhi"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.175.4017.82"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.97105102"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2004.04.009"},{"key":"e_1_2_6_68_1","first-page":"205","article-title":"Phthalate exposure and human semen quality in Shanghai: A cross\u2010sectional study","volume":"19","author":"Zhang YH","year":"2006","journal-title":"Biomed Environ Sci"}],"container-title":["Environmental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Ftox.21779","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.21779","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T14:42:49Z","timestamp":1696344169000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tox.21779"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4,4]]},"references-count":67,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["10.1002\/tox.21779"],"URL":"https:\/\/doi.org\/10.1002\/tox.21779","archive":["Portico"],"relation":{},"ISSN":["1520-4081","1522-7278"],"issn-type":[{"value":"1520-4081","type":"print"},{"value":"1522-7278","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4,4]]},"assertion":[{"value":"2011-08-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-03-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}