{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:58:59Z","timestamp":1790895539225,"version":"4.1.0"},"reference-count":79,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,3,10]],"date-time":"2015-03-10T00:00:00Z","timestamp":1425945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the Ministry of Science and Technology of the Republic of China (MOST)","award":["NSC-101-2320-B-040-019-MY2"],"award-info":[{"award-number":["NSC-101-2320-B-040-019-MY2"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Smoking increases the risk of cardiovascular disorders and leads to damage caused by inflammation and oxidative stress. The actin cytoskeleton is a key player in the response to inflammatory stimuli and is an early\n                    <jats:italic>target<\/jats:italic>\n                    of cellular\n                    <jats:italic>oxidative stress<\/jats:italic>\n                    . The purpose of this study was to investigate the changes in actin cytoskeleton dynamics in human endothelial\n                    <jats:italic>EA.hy926<\/jats:italic>\n                    cells exposed to cigarette smoke extract (CSE). Immunostaining revealed\n                    <jats:italic>that<\/jats:italic>\n                    CSE exposure resulted in modification of the\n                    <jats:italic>actin<\/jats:italic>\n                    cytoskeleton and led to cell rounding in a dose\u2010 and time\u2010dependent manner. In addition, the intracellular calcium concentration was increased by treatment with CSE. Pr\n                    <jats:italic>etreatment with antioxidants<\/jats:italic>\n                    (lipoic acid, glutathione,\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetyl cysteine, aminoguanidine,\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010tocopherol, and vitamin C) significantly\n                    <jats:italic>attenuated<\/jats:italic>\n                    the CSE\u2010induced actin cytoskeleton reorganization and cell rounding. Calcium ion chelators (EGTA, BAPTA\u2010AM AM) and a potent store\u2010operated calcium channel inhibitor (MRS 1845) also reduced CSE\u2010induced intracellular calcium changes and attenuated actin cytoskeleton reorganization and cell morphology change. Moreover, the CSE\u2010induced intracellular calcium increase was suppressed by pretreatment with the inositol trisphosphate receptor (IP3R) inhibitor xestospongin C, the phospholipase C (\n                    <jats:italic>PLC<\/jats:italic>\n                    )\n                    <jats:italic>inhibitor<\/jats:italic>\n                    U\u201073122, and the protein kinase C (PKC) inhibitor GF109203X. These results suggest that reactive oxygen species production and intracellular calcium increase play an essential role in CSE\u2010induced actin disorganization and cell rounding\n                    <jats:italic>through<\/jats:italic>\n                    a\n                    <jats:italic>PLC\u2013IP3\u2013PKC<\/jats:italic>\n                    signaling\n                    <jats:italic>pathway<\/jats:italic>\n                    . \u00a9 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1293\u20131306, 2016.\n                  <\/jats:p>","DOI":"10.1002\/tox.22133","type":"journal-article","created":{"date-parts":[[2015,3,11]],"date-time":"2015-03-11T00:40:54Z","timestamp":1426034454000},"page":"1293-1306","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["IP3 and calcium signaling involved in the reorganization of the actin cytoskeleton and cell rounding induced by cigarette smoke extract in human endothelial cells"],"prefix":"10.1002","volume":"31","author":[{"given":"Bo\u2010Hong","family":"Lin","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences Chung Shan Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Hsuan","family":"Tsai","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences Chung Shan Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong\u2010Kuei","family":"Lii","sequence":"additional","affiliation":[{"name":"Department of Nutrition China Medical University  Taichung Taiwan"},{"name":"Department of Health and Nutrition Biotechnology Asia University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsu\u2010Shing","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences Chung Shan Medical University  Taichung Taiwan"},{"name":"Department of Medical Research Chung Shan Medical University Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,10]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313975200"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1179\/135100007X162310"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-002-0011-4"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2010.12.001"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12123"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac801969f"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2012.03.004"},{"key":"e_1_2_5_9_1","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.1096\/fj.03-0312fje","article-title":"Disruption of vascular endothelial homeostasis by tobacco smoke: Impact on atherosclerosis","volume":"17","author":"Bernhard D","year":"2003","journal-title":"FASEB J"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1155"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-3623(96)00474-0"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.112.300157"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.05.006"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2010-1240"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2004.09.014"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.20441"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(01)00749-3"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2006.11.026"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-2888-2_16"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/01902140600710546"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.035402"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2013.11.013"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.05.017"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1ra00474c"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-012-0053-3"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2013.01.023"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a005579"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000127083.88549.58"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.4161\/chan.2.6.7055"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1316852110"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.022996"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.01.023"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2796.2002.01022.x"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx900008h"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0264224"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.12219SC"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-012-4147-4"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hyr141"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/ssq024"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302401200"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.96"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2012-0295OC"},{"key":"e_1_2_5_43_1","first-page":"313","article-title":"Effects of cigarette smoking on lipid peroxidation and antioxidant status in cancer patients from Western Nepal","volume":"12","author":"Nagamma T","year":"2011","journal-title":"Asian Pac J Cancer Prev"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.061051"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5615"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.7.4668"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2005.06.028"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106183108"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1993.tb39148.x"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2010.05.002"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/15376510903104224"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.545137"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-05-2015"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.4.1628-1639.2004"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3412"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2012.08.003"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.166678"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00385.2010"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1402515111"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-8-104"},{"key":"e_1_2_5_61_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.19.5.3091"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M512885200"},{"key":"e_1_2_5_63_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.2887"},{"key":"e_1_2_5_64_1","doi-asserted-by":"publisher","DOI":"10.1080\/10739680600930347"},{"key":"e_1_2_5_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/54.1.104"},{"key":"e_1_2_5_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-014-9032-y"},{"key":"e_1_2_5_67_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2003.50069"},{"key":"e_1_2_5_68_1","unstructured":"U.S. Department of Health and Human Services.2010. How tobacco smoke causes disease: The biology and behavioral basis for smoking\u2010attributable disease: A report of the surgeon general. Atlanta GA: U.S. Department of Health and Human Services Centers for Disease Control and Prevention National Center for Chronic Disease Prevention and Health Promotion Office on Smoking and Health."},{"key":"e_1_2_5_69_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph6020445"},{"key":"e_1_2_5_70_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.90461.2008"},{"key":"e_1_2_5_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-14-45"},{"key":"e_1_2_5_72_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045615"},{"key":"e_1_2_5_73_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-11-0132"},{"key":"e_1_2_5_74_1","doi-asserted-by":"crossref","first-page":"8396","DOI":"10.1016\/S0021-9258(18)42458-1","article-title":"Modification of chromaffin cells with pertussis toxin or N\u2010ethylmaleimide lowers cytoskeletal F\u2010actin and enhances Ca(2+)\u2010dependent secretion","volume":"267","author":"Wu YN","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_5_75_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1221538110"},{"key":"e_1_2_5_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2009.49"},{"key":"e_1_2_5_77_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000187473.85419.3e"},{"key":"e_1_2_5_78_1","first-page":"709","article-title":"Cigarette smoke extract activates PKC isoforms and down\u2010regulates the expressions of potassium channels BK(Ca) and Kv1.5 in rat bronchial smooth muscle cells","volume":"60","author":"Ye H","year":"2008","journal-title":"Sheng Li Xue Bao"},{"key":"e_1_2_5_79_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00048.2006"},{"key":"e_1_2_5_80_1","doi-asserted-by":"crossref","unstructured":"YuLX DzikovskiBG FreedJH.2012.A protocol for detecting and scavenging gas\u2010phase free radicals in mainstream cigarette smoke.J Vis Exp59:e3406.","DOI":"10.3791\/3406-v"}],"container-title":["Environmental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Ftox.22133","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.22133","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T06:25:17Z","timestamp":1696487117000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tox.22133"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,10]]},"references-count":79,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1002\/tox.22133"],"URL":"https:\/\/doi.org\/10.1002\/tox.22133","archive":["Portico"],"relation":{},"ISSN":["1520-4081","1522-7278"],"issn-type":[{"value":"1520-4081","type":"print"},{"value":"1522-7278","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,10]]},"assertion":[{"value":"2014-09-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}