{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T19:12:20Z","timestamp":1784574740056,"version":"3.55.0"},"reference-count":57,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T00:00:00Z","timestamp":1487203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","award":["41\u201084\/2012(SR) dated 11th July, 2012"],"award-info":[{"award-number":["41\u201084\/2012(SR) dated 11th July, 2012"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Excess iodine induced public health problems are now emerging in many iodine sufficient regions for indiscriminate intake of iodine through various iodized products. It has been reported that excess iodine can disrupt overall male reproductive physiology by generating oxidative stress in the testis. However, information on the possible effect of iodine in excess on spermatozoa found less. In the present investigation flow cytometric techniques and scanning electron microscopy (SEM) have been used to study the spermatozoal functional as well as structural status under the influence of excess iodine; generation of ROS in the spermatozoa as evident by DCFDA, altered acrosomal integrity as observed by fluorescence lectin staining method and depolarized mitochondrial membrane potential (\u0394<jats:italic>\u03a8<\/jats:italic><jats:sub>m<\/jats:sub>) noticed by JC\u20101 staining. Ultrastructure of seminiferous tubule after excess iodine exposure indicated severe deterioration of seminiferous tubular surface architecture. Significant increase in spermatozoal DNA fragmentation and apoptotic sperms were found by acridine orange and Annexin V, respectively, however the plasma membrane integrity\/viability was decreased as evident by propidium iodide staining in various incremental doses and durations under iodine excess. The study reveals that excess iodine could cause apoptosis of spermatozoal cells by inducing ROS that ultimately affects male fertility potential.<\/jats:p>","DOI":"10.1002\/tox.22405","type":"journal-article","created":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T06:32:43Z","timestamp":1487226763000},"page":"1823-1835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Influence of iodine in excess on seminiferous tubular structure and epididymal sperm character in male rats"],"prefix":"10.1002","volume":"32","author":[{"given":"Amar K","family":"Chandra","sequence":"first","affiliation":[{"name":"Endocrinology and Reproductive Physiology Laboratory, Department of Physiology University College of Science and Technology, University of Calcutta 92, Acharya Prafulla Chandra Road Kolkata West Bengal 700 009 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arijit","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Endocrinology and Reproductive Physiology Laboratory, Department of Physiology University College of Science and Technology, University of Calcutta 92, Acharya Prafulla Chandra Road Kolkata West Bengal 700 009 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.90.10.1633"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-009-0081-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"343","DOI":"10.17221\/5845-VETMED","article-title":"Iodine toxicity in ruminants","volume":"47","author":"Paul\u00edkov\u00e1 I","year":"2002","journal-title":"Vet Med Czech."},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-011-9300-x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/15.2.366"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.305.6854.609"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.309.6952.476"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910590108"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-015-0581-3"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2016.10.006"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-007-9569-4"},{"key":"e_1_2_6_13_1","first-page":"161","article-title":"Effects of chronic administration of high doses of potassium iodide on iodine metabolism in the rat thyroid gland","volume":"52","author":"Lupachik SV","year":"2006","journal-title":"Biomed Khim."},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2009.08.010"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.14-0421"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080320105"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0890-6238(00)00113-1"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod53.2.276"},{"key":"e_1_2_6_19_1","volume-title":"Methods in Cell Biology. 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