{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:33:05Z","timestamp":1788571985132,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T00:00:00Z","timestamp":1508803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T00:00:00Z","timestamp":1508803200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    2,3,5,4\u2032\u2010Tetrahydroxystilbene\u20102\u2010O\u2010\u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010glucoside (THSG) is an active compound extracted from\n                    <jats:italic>Polygonum multiflorum<\/jats:italic>\n                    Thunb. This herb and radix Polygoni Multiflori preparata have been used to treat arteriosclerosis, hyperlipidemia, hypercholesterolemia, and diabetes for thousands of years. This study aimed to investigate the protective effects of THSG in an Adriamycin (AD)\u2010induced focal segmental glomerulosclerosis (FSGS) mouse model and the underlying mechanisms in an in vitro system. Mice were treated with THSG (2.5 and 10 mg\/kg, oral gavage) for 24 consecutive days. On the third day, mice were intravenously given a single dose of AD (10 mg\/kg). At the end of the experiment, plasma and kidney samples were harvested to evaluate the therapeutic effects of THSG. The potential mechanisms of THSG in protecting against AD\u2010induced cytotoxicity were examined using a real\u2010time polymerase chain reaction, immunoblots, lactate dehydrogenase assay, and a cellular oxidized\u2010thiol detection system in a mouse mesangial cell line. In this study, THSG showed concentration\u2010dependent protective effects in ameliorating the progression of AD\u2010induced FSGS. THSG suppressed albuminuria and hypercholesterolemia and reduced the status of lipid peroxidation in urine, plasma, and kidney tissue samples. Furthermore, THSG protected against podocyte damage, reduced renal fibrotic gene expressions, and alleviated the severity of glomerulosclerosis. Treatment of mouse mesangial cells with THSG induced nuclear factor erythroid\u2010derived 2\u2010like 2 (Nrf2) nuclear translocation, increased heme oxygenase\u20101 and NAD(P)H:quinone oxidoreductase (NQO)\u20101 gene expressions, and reduced cellular thiol oxidation and resistance to AD\u2010induced cytotoxicity. Silencing Nrf2 and its repressor protein, Kelch\u2010like ECH\u2010associated protein 1 (Keap1), abolished these protective effects of THSG. In conclusion, THSG can play a protective role in ameliorating the progression of FSGS in a mouse model through activation of the Nrf2\u2010Keap1 antioxidant pathway. Although a well\u2010designed therapeutic study is needed, THSG may be applied to manage chronic kidney disease.\n                  <\/jats:p>","DOI":"10.1002\/tox.22496","type":"journal-article","created":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T06:52:43Z","timestamp":1508827963000},"page":"72-82","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["The natural compound 2,3,5,4\u2032\u2010tetrahydroxystilbene\u20102\u2010O\u2010\u03b2\u2010\n                    <scp>d<\/scp>\n                    glucoside protects against adriamycin\u2010induced nephropathy through activating the Nrf2\u2010Keap1 antioxidant pathway"],"prefix":"10.1002","volume":"33","author":[{"given":"En\u2010Yuan","family":"Lin","sequence":"first","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine Taipei Medical University Taipei Taiwan"},{"name":"Department of Neurosurgery Taipei Medical University Hospital, Taipei Medical University Taipei Taiwan"},{"name":"Division of Neurosurgery Department of Surgery, Taiwan Adventist Hospital, Taipei  Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uyanga","family":"Bayarsengee","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine Taipei Medical University Taipei Taiwan"},{"name":"Department of Internal Medicine Shastin Central Hospital in Ulaanbaatar  Mongolia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Chiung","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Pharmacognosy, College of Pharmacy Taipei Medical University Taipei Taiwan"},{"name":"School of Pharmacy, College of Pharmacy Taipei Medical University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung\u2010Hsiao","family":"Chiang","sequence":"additional","affiliation":[{"name":"Graduate Institute of 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