{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:18:37Z","timestamp":1790140717361,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T00:00:00Z","timestamp":1510185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008641","name":"Chung Shan Medical University","doi-asserted-by":"publisher","award":["NCHU\u2010CSMU\u201010507"],"award-info":[{"award-number":["NCHU\u2010CSMU\u201010507"]}],"id":[{"id":"10.13039\/501100008641","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Oral submucous fibrosis (OSF) has been recognized as one of the oral potentially malignant disorders. Areca nut chewing is implicated in this pathological fibrosis, and it causes chronic inflammation and persistent activation of myofibroblasts. As yet, existing treatments only provide temporary symptomatic relief and there is a lack of an effective intervention to cure OSF. Therefore, development of approaches to ameliorate myofibroblast activities becomes a crucial objective to prevent the malignant progression of OSF. In this study, we examined the inhibitory effect of glabridin, an isoflavane extracted from licorice root, on the myofibroblast characteristics in human fibrotic buccal mucosal fibroblasts (fBMFs). Our results showed that myofibroblast activities, including collagen gel contractility, migration, invasion and wound healing abilities were reduced after exposure of glabridin in a dose\u2010dependent manner. Most importantly, we demonstrated that the arecoline\u2010induced myofiroblast activities were abolished by glabridin treatment. Additionally, the expression of the myofibroblast marker \u03b1\u2010smooth muscle actin and other fibrogenic marker, type I collagen, in fBMFs were dose\u2010dependently downregulated. Moreover, we showed that the production of TGF\u2010\u03b2 was suppressed by glabridin in fBMFs and the protein expression of phospho\u2010Smad2 was decreased as well. In summary, our data suggested that glabridin repressed the myofibroblast features in fBMFs via TGF\u2010\u03b2\/Smad2 signaling pathway. Glabridin also prevented the arecoline\u2010increased myofibroblast activities, and could serve as a natural anti\u2010fibrosis compound for OSF.<\/jats:p>","DOI":"10.1002\/tox.22512","type":"journal-article","created":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T06:24:38Z","timestamp":1510208678000},"page":"248-255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Glabridin inhibits the activation of myofibroblasts in human fibrotic buccal mucosal fibroblasts through TGF\u2010\u03b2\/smad signaling"],"prefix":"10.1002","volume":"33","author":[{"given":"Ping\u2010Hui","family":"Lee","sequence":"first","affiliation":[{"name":"School of Dentistry Chung Shan Medical University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Ming","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Graduate Institute of Biomedical Sciences, School of Medicine China Medical University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Ling","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Institute of Oral Sciences, Chung Shan Medical University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Wen","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Dentistry Chung Shan Medical University Taichung Taiwan"},{"name":"Department of Dentistry Chung Shan Medical University Hospital Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3200-7552","authenticated-orcid":false,"given":"Pin Ju","family":"Chueh","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, National Chung Hsing University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Feng","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Dentistry Chung Shan Medical University Taichung Taiwan"},{"name":"Department of Dentistry Chung Shan Medical University Hospital Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Wen","family":"Liao","sequence":"additional","affiliation":[{"name":"School of Dentistry Chung Shan Medical University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Chia","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Dentistry Chung Shan Medical University Taichung Taiwan"},{"name":"Institute of Oral Sciences, Chung Shan Medical University Taichung Taiwan"},{"name":"Department of Dentistry Chung Shan Medical University Hospital Taichung 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