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CCl<jats:sub>4<\/jats:sub>\u2010intduced model of liver fibrosis in Wistar rats is most widely used model to study chemopreventive studies. CCl<jats:sub>4<\/jats:sub>\u2010intoxication significantly increased serum aminotransferases and total cholesterol these effects were prevented by cotreatment with D\u2010Limonene. Also, CCl<jats:sub>4<\/jats:sub>\u2010intoxication caused depletion of glutathione and other antioxidant enzymes while D\u2010Limonene preserved them within normal values. Hydroxyproline and malondialdehyde content was increased markedly by CCl<jats:sub>4<\/jats:sub> treatment while D\u2010Limonene prevented these alterations. Levels of TNF\u2010\u03b1, TGF\u2010\u03b2, and \u03b1\u2010SMA were also assessed; CCl<jats:sub>4<\/jats:sub> increased the expression of \u03b1\u2010SMA, NF\u2010\u03baB and other downstream inflammatory cascade while D\u2010Limonene co\u2010treatment inhibited them. Collectively these findings indicate that D\u2010Limonene possesses potent antifibrotic effect which may be attributed to its antioxidant and anti\u2010inflammatory properties.<\/jats:p>","DOI":"10.1002\/tox.22523","type":"journal-article","created":{"date-parts":[[2017,12,18]],"date-time":"2017-12-18T11:03:14Z","timestamp":1513594994000},"page":"361-369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Antifibrotic effects of D\u2010limonene (5(1\u2010methyl\u20104\u2010[1\u2010methylethenyl]) cyclohexane) in CCl<sub>4<\/sub> induced liver toxicity in Wistar rats"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6360-8457","authenticated-orcid":false,"given":"Sheikh Bilal","family":"Ahmad","sequence":"first","affiliation":[{"name":"Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences &amp; Animal Husbandry Sheri Kashmir University of Agricultural Science &amp; 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