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Hinokitiol, a natural tropolone derived from Chamacyparis taiwanensis, has been reported to improve oral lichen planus and inhibit various cancer cells. Here, we showed that hinokitiol reduced the myofibroblast activities in fBMFs and prevented the arecoline\u2010induced transdifferentiation. Treatment of hinokitiol dose\u2010dependently downregulated the myofibroblast markers as well as various EMT transcriptional factors. In particular, we identified that Snail was able to bind to the E\u2010box in the \u03b1\u2010SMA promoter. Our data suggested that exposure of fBMFs to hinokitiol mitigated the hallmarks of myofibroblasts, while overexpression of Snail eliminated the effect of hinokitiol. These findings revealed that the inhibitory effect of hinokitiol on myofibroblasts was mediated by repression of \u03b1\u2010SMA via regulation of Snail and showed the anti\u2010fibrotic potential of hinokitiol in the treatment of OSF.<\/jats:p>","DOI":"10.1002\/tox.22531","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T13:36:38Z","timestamp":1515764198000},"page":"454-462","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Hinokitiol ablates myofibroblast activation in precancerous oral submucous fibrosis by targeting Snail"],"prefix":"10.1002","volume":"33","author":[{"given":"Hui\u2010Wen","family":"Yang","sequence":"first","affiliation":[{"name":"School of Dentistry, Chung Shan Medical University Taichung Taiwan"},{"name":"Department of Dentistry Chung Shan Medical University Hospital Taichung 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