{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T05:05:21Z","timestamp":1793423121914,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,2,15]],"date-time":"2018-02-15T00:00:00Z","timestamp":1518652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Fundamental Research Funds for Central Universities, HUST","award":["2011TS113"],"award-info":[{"award-number":["2011TS113"]}]},{"name":"Hubei Province Health and Family Planning Scientific Research Project","award":["WJ2015MB177"],"award-info":[{"award-number":["WJ2015MB177"]}]},{"name":"Hubei Provincial Natural Science Foundation of China","award":["3013CKD015"],"award-info":[{"award-number":["3013CKD015"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Toxicity of lead on male reproductive functions has raised wide public concern as environmental lead contamination remains common worldwide. Conflicting and controversial data are available regarding effects of lead on male fertility. More importantly, our knowledge on effects of lead on sperm DNA integrity is significantly limited. Thus, further studies should focus on this issue. In the current study, adult male mice were exposed to a series of lead acetate concentrations in drinking water for six weeks. Following administration, lead levels in blood, testicles, and epididymis were measured, and potential changes in morphology of testis and epididymis due to lead exposure were identified. We also analyzed sperm parameters, including sperm density, viability, motility, and morphology, to evaluate quality of sperm collected from epididymis. Especially, hypothetical influence of lead on sperm DNA integrity was also evaluated by terminal deoxynucleotidyltransferase\u2010mediated dUTP\u2010biotin nick end labeling, alkaline comet assay, and sperm chromatin structure assay. Lead exposure possibly exerted no effect on growth of mice because these animals acquired similar body weight gain during the experimental period. However, high lead concentrations (0.5% and 1%) in drinking water affected sperm motility and increased percentage of spermatozoa with abnormal morphology. In groups treated with 0.25%, 0.5%, and 1% lead acetate, percentages of sperm cells showing DNA breaks and chromatin structure damage significantly increased. Altogether, lead exposure not only exhibits adverse effects on sperm physiological parameters, but also impairs DNA structure and integrity. These effects may lead to significant decline in male fertility.<\/jats:p>","DOI":"10.1002\/tox.22545","type":"journal-article","created":{"date-parts":[[2018,2,15]],"date-time":"2018-02-15T04:48:39Z","timestamp":1518670119000},"page":"594-602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["Lead exposure reduces sperm quality and DNA integrity in mice"],"prefix":"10.1002","volume":"33","author":[{"given":"Cuiling","family":"Li","sequence":"first","affiliation":[{"name":"Department of Medical Ultrasound, Guangzhou First People's Hospital Guangzhou China"},{"name":"Institute of Family Planning Research, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Family Planning Research, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Family Planning Research, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology Wuhan Children Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Urology First College of Clinical Medical Science\/Yichang Central People's Hospital, Three Gorges University Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Tianyou Hospital affiliated to Wuhan University of Science &amp; Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5213-1024","authenticated-orcid":false,"given":"Biao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ucl.2013.08.008"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03411.x"},{"key":"e_1_2_8_4_1","unstructured":"AbadinH AshizawaA StevensYW LladosF DiamondG SageG et al.Toxicological Profile for Lead. 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