{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T08:27:20Z","timestamp":1751358440392,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T00:00:00Z","timestamp":1536192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n<jats:italic>Clinacanthus nutans<\/jats:italic> has been used as herbal medicine with antidiabetic, blood pressure lowering, and diuretic properties in Singapore, Thailand, and Malaysia. The in vitro cellular study showed the chloroform extract possessed significant cytotoxicity against leukemia K562 and lymphoma Raji cells. The clinical study reported that administration of plant could treat or prevent relapse in 12 cancer patients. However, detailed mechanism of the anticancer effects and chemical profiles are not thoroughly studied. The chemical study did show that the acetone extract (MHA) exerted the highest antiproliferative effect on human leukemia MOLT\u20104 cells and lymphoma SUP\u2010T1 cells in dose\u2010dependent cytotoxicity. We found that the use of MHA increased apoptosis by 4.28%\u201043.65% and caused disruption of mitochondrial membrane potential (MMP) by 11.79%\u201026.93%, increased reactive oxygen species (ROS) by 19.54% and increased calcium ion by 233.83%, as demonstrated by annexin\u2010V\/PI, JC\u20101, H<jats:sub>2<\/jats:sub>DCFDA, and Flou\u20103 staining assays, respectively. MHA\u2010induced ER stress was confirmed by increase expression of CHOP and IRE\u20101\u03b1 with western blotting assay. In conclusion, we identified good bioactivity in <jats:italic>Clinacanthus nutans<\/jats:italic> and recognize its potential effect on cancer therapy, but further research is needed to determine the use of the plant.<\/jats:p>","DOI":"10.1002\/tox.22629","type":"journal-article","created":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T09:11:04Z","timestamp":1536225064000},"page":"1229-1236","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Chemical evaluation and cytotoxic mechanism investigation of <i>Clinacanthus nutans<\/i> extract in lymphoma SUP\u2010T1 cells"],"prefix":"10.1002","volume":"33","author":[{"given":"Mei\u2010Chin","family":"Lu","sequence":"first","affiliation":[{"name":"Graduate Institute of Marine Biology National Dong Hwa University  Hualien Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsung\u2010Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Food Science, Agricultural College National Pingtung University of Science and Technology  Neipu Pingtung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Chun","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Technology, National Taiwan University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7181-2183","authenticated-orcid":false,"given":"Pao\u2010Chuan","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Food Science, Agricultural College National Pingtung University of Science and Technology  Neipu Pingtung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yung\u2010Lin","family":"Chu","sequence":"additional","affiliation":[{"name":"International Master's Degree Program in Food Science, International College National Pingtung University of Science and Technology  Neipu Pingtung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786410802393548"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.01.059"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.11.050"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2011.1780"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2015.1087042"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22544"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgp044"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctim.2010.12.003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221-1691(13)60064-7"},{"issue":"12","key":"e_1_2_6_11_1","first-page":"1737","article-title":"Efficacy and side effect of continuous intra\u2010arterial infusion of high\u2010dose 5\u2010FU for liver metastases of colorectal cancer","volume":"26","author":"Kurokawa Y","year":"1999","journal-title":"Gan to kagaku ryoho Cancer Chemother."},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.4172\/jpb.1000363"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf5059304"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf403241s"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2011.02876.x"},{"issue":"5","key":"e_1_2_6_17_1","first-page":"1243","article-title":"Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria\u2010dependent signal pathways","volume":"37","author":"Kuo JH","year":"2010","journal-title":"Int J Oncol."},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2016.4278"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9180"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.3945\/an.116.012211"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0353.2003.03711.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/462751"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf301298y"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22396"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.4103\/2225-4110.114895"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcme.2014.11.026"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2015.00167"},{"issue":"11","key":"e_1_2_6_28_1","first-page":"650","article-title":"Express of plasma ROS, SOD and GSH\u2010PX in patients with nasopharyngeal carcinoma","volume":"17","author":"Zhang M","year":"2003","journal-title":"J Clin Otorhinolaryngol."},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.308"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010203213500"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2017.1247884"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22030437"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109950200"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.108.060780"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2010.11.018"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057604"}],"container-title":["Environmental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Ftox.22629","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.22629","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T07:24:38Z","timestamp":1694849078000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tox.22629"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,6]]},"references-count":35,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1002\/tox.22629"],"URL":"https:\/\/doi.org\/10.1002\/tox.22629","archive":["Portico"],"relation":{},"ISSN":["1520-4081","1522-7278"],"issn-type":[{"type":"print","value":"1520-4081"},{"type":"electronic","value":"1522-7278"}],"subject":[],"published":{"date-parts":[[2018,9,6]]},"assertion":[{"value":"2018-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}