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In this study, we investigated anti\u2010metastasis mechanisms of chrysin in human melanoma cancer A375.S2 cells in vitro. Under sub\u2010lethal concentrations of chrysin (0, 5, 10, and 15\u2009\u03bcM) which inhibits cell mobility, migration and invasion of A375.S2 cells that were assayed by wound healing and Transwell filter. That chrysin inhibited MMP\u20102 activity in A375.S2 cells was investigated by gelatin zymography assay. Western blotting was used to examine protein expression and results indicated that chrysin inhibited the expression of GRB2, SOS\u20101, PKC, p\u2010AKT (Thr308), NF\u2010\u03baBp65, and NF\u2010\u03baBp50 at 24 and 48\u2009hours treatment, but only at 10\u201015\u2009\u03bcM of chrysin decreased Ras, PI3K, p\u2010c\u2010Jun, and Snail only at 48\u2009hours treatment and only decrease p\u2010AKT(Ser473) at 24\u2009hours treatment. Furthermore, chrysin (5\u201015\u2009\u03bcM) decreased the expression of uPA, N\u2010cadherin and MMP\u20101 at 24 and 48\u2009hours treatment but only decreased MMP\u20102 and VEGF at 48\u2009hours treatment at 10\u201015\u2009\u03bcM and 5\u201015\u2009\u03bcM of chrysin, respectively, however, increased E\u2010cadherin at 5\u201015\u2009\u03bcM treatment. Results of confocal laser microscopy systems indicated that chrysin inhibited expression of NF\u2010\u03baBp65 in A375.S2 cells. Based on these observations, we suggest that chrysin can be used in anti\u2010metastasis of human melanoma cells in the future.<\/jats:p>","DOI":"10.1002\/tox.22697","type":"journal-article","created":{"date-parts":[[2018,12,22]],"date-time":"2018-12-22T10:54:07Z","timestamp":1545476047000},"page":"434-442","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Chrysin inhibit human melanoma A375.S2 cell migration and invasion via affecting MAPK signaling and NF\u2010\u03baB signaling pathway in vitro"],"prefix":"10.1002","volume":"34","author":[{"given":"Hsin\u2010Yu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biological Science and Technology China Medical University  Taichung Taiwan"},{"name":"Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources China Medical University  Taichung 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