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The subject of this study is to evaluate the antiproliferation effects on oral cancer cells by <jats:italic>Nepenthes<\/jats:italic> plants using ethyl acetate extract of <jats:italic>Nepenthes adrianii<\/jats:italic> x <jats:italic>clipeata<\/jats:italic> (EANA). Cell viability was detected using MTS assay. Its detailed mechanisms including cell cycle, apoptosis, oxidative stress, and DNA damage were explored by flow cytometry or western blotting. For 24\u2009hours EANA treatment, five kinds of oral cancer cells (CAL 27, Ca9\u201022, OECM\u20101, HSC\u20103, and SCC9) show IC<jats:sub>50<\/jats:sub> values of cell viability ranging from 8 to 17\u2009\u03bcg\/mL but the viability of normal oral cells (HGF\u20101) remains over 80%. Subsequently, CAL 27 and Ca9\u201022 cells with high sensitivity to EANA were chosen to investigate the detailed mechanism. EANA displays the time course and concentration effects for inducing apoptosis based on flow cytometry (subG1 and annexin V analyses) and western blotting [cleaved poly (ADP\u2010ribose) polymerase (c\u2010PARP)]. Oxidative stress and DNA damage were induced by EANA treatments in oral cancer cells through reactive oxygen species (ROS), mitochondrial membrane potential disruption, mitochondrial superoxide, and \u03b3H2AX. All these changes of EANA treatments in oral cancer cells were reverted by the ROS scavenger <jats:italic>N<\/jats:italic>\u2010acetylcysteine pretreatment. Therefore, EANA induces preferential killing, apoptosis, and DNA damage against oral cancer cells through oxidative stress.<\/jats:p>","DOI":"10.1002\/tox.22748","type":"journal-article","created":{"date-parts":[[2019,6,3]],"date-time":"2019-06-03T12:16:33Z","timestamp":1559564193000},"page":"891-901","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Ethyl acetate extract of <i>Nepenthes adrianii<\/i> x <i>clipeata<\/i> induces antiproliferation, apoptosis, and DNA damage against oral cancer cells through oxidative stress"],"prefix":"10.1002","volume":"34","author":[{"given":"Jen\u2010Yang","family":"Tang","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Radiation Oncology, College of Medicine Kaohsiung Medical University  Kaohsiung Taiwan"},{"name":"Department of Radiation Oncology Kaohsiung Medical University Hospital  Kaohsiung 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