{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T22:31:44Z","timestamp":1789079504795,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,8,9]],"date-time":"2019-08-09T00:00:00Z","timestamp":1565308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The objective of the present study was to explore the effects of environmental chemicals, such as methyl mercury, paraquat, and bisphenol A, on cell proliferation and apoptosis, as well as the expression levels of neuronal differentiation\u2010related genes in neural stem cells (NSCs). NSCs originated from human umbilical cord blood (HUCB\u2010NSCs) were used as cell models in the current study. CCK\u20108 and flow cytometry experiments were performed to assess the effects of methyl mercury, paraquat, and bisphenol A on the proliferation and apoptosis of HUCB\u2010NSCs at different processes, including proliferation and differentiation stages. The expressions of neuronal differentiation\u2010related genes were determined by reverse transcription\u2010polymerase chain reaction and western blot analysis. The results showed that methyl mercury, paraquat, and bisphenol A treatments significantly inhibited cell proliferation and induced cell apoptosis in HUCB\u2010NSCs, as well as decreased the expressions of Oct4, Gdf3, and Sox1, whereas increased Pax6 and Ngn1 expressions at both mRNA and protein levels. In conclusion, this study demonstrates that environmental chemicals can impair the proliferation and differentiation of NSCs, which may cause abnormal development of the nervous system.<\/jats:p>","DOI":"10.1002\/tox.22829","type":"journal-article","created":{"date-parts":[[2019,8,10]],"date-time":"2019-08-10T02:24:29Z","timestamp":1565403869000},"page":"1285-1291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Effects of environmental chemicals on the proliferation and differentiation of neural stem cells"],"prefix":"10.1002","volume":"34","author":[{"given":"Min","family":"Huang","sequence":"first","affiliation":[{"name":"The Department of Occupational and Environmental Health\/Lab of Molecular Toxicology, School of Public Health and Management Ningxia Medical University  Yinchuan 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