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We first used an IGF\u2010II analog Leu27IGF\u2010II, which specifically activates IGF2R signaling cascades and induces H9c2 cardiomyoblast cell hypertrophy. However, Danshen extract completely inhibited Leu27IGF\u2010II\u2010induced cell size increase, ANP and BNP hypertrophic marker expression, and IGF2R induction. We also observed that Danshen extract inhibited calcineurin protein expression and NFAT3 nuclear translocation, leading to suppression of Leu27IGF\u2010II\u2010induced cardiac hypertrophy. Moreover, the anti\u2010Leu27IGF\u2010II\u2010IGF2R signaling effect of Danshen was totally reversed by ICI, which suggest the cardio protective effect of Danshen is mediated through estrogen receptors. Our study suggests that, Danshen exerts estrogenic activity, and thus, it could be used as a selective ER modulator in IGFIIR induced hypertrophy model.<\/jats:p>","DOI":"10.1002\/tox.22940","type":"journal-article","created":{"date-parts":[[2020,5,16]],"date-time":"2020-05-16T14:46:27Z","timestamp":1589640387000},"page":"1043-1049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Danshen (<scp><i>Salvia miltiorhiza<\/i><\/scp>) inhibits Leu27 <scp>IGF\u2010II<\/scp>\u2010induced hypertrophy in H9c2 cells"],"prefix":"10.1002","volume":"35","author":[{"given":"Tsung\u2010Jung","family":"Ho","sequence":"first","affiliation":[{"name":"Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002  Taiwan"},{"name":"Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien 97002  Taiwan"},{"name":"School of Post\u2010Baccalaure\u2010ate Chinese Medicine, College of 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