{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T04:11:26Z","timestamp":1780632686384,"version":"3.54.1"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T00:00:00Z","timestamp":1595203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Central University of Punjab","award":["GP-25"],"award-info":[{"award-number":["GP-25"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Being one of the notorious weed <jats:italic>P. hysterophorus<\/jats:italic> has invaded almost every part India and is the lead cause of skin allergies and severe dermatitis among farmers and rural population. It is an invasive obnoxious weed capable of surviving extreme environmental conditions and various parts of this plant are reported to cause severe contact allergies in humans due to the presence of high concentrations of toxic sesquiterpene lactones viz. parthenin. It can stimulate numerous cellular and immune responses that may translate into Oxidative stress, allergies, and inflammation. The effect of <jats:italic>P. hysterophorus<\/jats:italic> flower extract was evaluated on cell viability, oxidative stress and inflammation in A549 lung cancer cell line by spectrophotometric and reverse <jats:styled-content style=\"fixed-case\">transcriptase\u2010polymerase<\/jats:styled-content> chain reaction methods. Schrodinger software based docking was performed for possible interactions studies. The A549 cells treated with <jats:italic>P. hysterophorus<\/jats:italic> flower extract favors increase in cell viability, reactive oxygen species generation. The mRNA expression of proinflammatory cytokines such as IFN\u2010\u03b3, TNF\u2010\u03b1, and IL\u20101\u03b2 was significantly increased whereas no change in IL\u201018 expression was observed. Significant increase in protein expression of NF\u2010\u03baB was observed, suggesting the role of NF\u2010\u03baB signalling in allergic responses. The docking studies demonstrated the potential interaction between Parthenin and NF\u2010\u03baB\/IL\u20101\u03b2\/IL\u201018 suggesting their activation leading to inflammation. The current study emphasize that <jats:italic>P. hysterophorus<\/jats:italic> mediates oxidative stress, and inflammatory process via alterations in expression of proinflammatory cytokines such as IL\u20101\u03b2, IFN\u2010\u03b3 through NF\u2010\u03baB activation which was also confirmed in docking studies. Cellular and molecular mechanisms involved in pathogenesis of allergic\/chronic inflammation and severe dermatitis need to be further investigated to identify specific binding partners responsible for severe inflammation which can provide some leads in developing effective targets against severe dermatitis and skin allergies.<\/jats:p>","DOI":"10.1002\/tox.22989","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T11:13:41Z","timestamp":1595243621000},"page":"1241-1250","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp><i>Parthenium hysterophorus<\/i><\/scp> mediated inflammation and hyper\u2010responsiveness via <scp>NF\u2010\u03baB<\/scp> pathway in human <scp>A549<\/scp> lung cancer cell line"],"prefix":"10.1002","volume":"35","author":[{"given":"Mandeep","family":"Kaur","sequence":"first","affiliation":[{"name":"Department of Human Genetics and Molecular Medicine School of Health Sciences, Central University of Punjab  Bathinda India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunj Bihari","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Basic and Applied Sciences, Central University of Punjab  Bathinda India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shweta","family":"Thakur","sequence":"additional","affiliation":[{"name":"Department of Zoology School of Basic and Applied Sciences, Central University of Punjab  Bathinda India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukhchain","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Basic and Applied Sciences, Central University of Punjab  Bathinda India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5923-3384","authenticated-orcid":false,"given":"Monisha","family":"Dhiman","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Basic and Applied Sciences, Central University of Punjab  Bathinda India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07204"},{"issue":"2","key":"e_1_2_8_3_1","first-page":"131","article-title":"Aeroallergens in clinical practice of allergy in India. 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