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Silver nanocolloid (SNC) is known to induce vascular malformation in the medaka embryo. We focused on the development of the central arteries (CtAs) in the hindbrain of Japanese medaka. The CtAs and the basilar artery from which they branch are essential for transporting the blood and nutrients necessary to support the hindbrain parenchyma and the development of the pons and cerebellum from the hindbrain. We exposed medaka embryos at developmental stage 21 (6 somite stage), to 0, 0.5, 5, or 10 mg\/L SNC and evaluated hatching rate, number of thrombi per embryo, head size (length and width), body length, and angiogenesis. Although all SNC\u2010exposed embryos hatched, their head size and body length were small in comparison to controls; in addition, the number of thrombi in the head increased and head size and body length decreased as the SNC concentration increased. To evaluate vasculogenic abnormalities, we performed whole\u2010mount in situ hybridization using a vascular marker (eg, <jats:italic>fl7<\/jats:italic>) and visualized the CtAs in medaka embryos. In control embryos, CtAs started to sprout at stage 32 (somite completion stage) and their extension was complete by stage 35 (pectoral fin blood circulation stage). In contrast, CtAs failed to sprout in SNC\u2010exposed embryos, and thrombi were present. Furthermore, qRT\u2010PCR analysis showed that SNC significantly suppressed the <jats:italic>egfl7<\/jats:italic> expression level at stage 35. Together, our findings suggest that SNC induced decreased developments of head and body in medaka embryos due to insufficient angiogenesis and hindbrain vascular formation.<\/jats:p>","DOI":"10.1002\/tox.23047","type":"journal-article","created":{"date-parts":[[2020,10,24]],"date-time":"2020-10-24T13:24:29Z","timestamp":1603545869000},"page":"417-424","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Silver nanocolloid affects hindbrain vascular formation during medaka embryogenesis"],"prefix":"10.1002","volume":"36","author":[{"given":"Tomomi","family":"Matsukura","sequence":"first","affiliation":[{"name":"Graduate School of Life Sciences Toyo University  Itakura Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chisato","family":"Kataoka","sequence":"additional","affiliation":[{"name":"Japan Society for the Promotion of Science  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohei","family":"Kawana","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Toyo University  Itakura Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Misato","family":"Fujita","sequence":"additional","affiliation":[{"name":"Faculty of Science Kanagawa University  Hiratsuka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0148-2107","authenticated-orcid":false,"given":"Shosaku","family":"Kashiwada","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences Toyo University  Itakura Japan"},{"name":"Department of Life Sciences Toyo University  Itakura Japan"},{"name":"Research Centre for Life and Environmental Sciences Toyo University  Itakura Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3762\/bjnano.6.181"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/es7029637"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/19\/25\/255102"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200801716"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/es2045647"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2017.10.028"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.08.008"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2011.05.1033"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/17435390.2016.1214763"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00244-014-0091-x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2004.03.012"},{"issue":"3","key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1002\/dvdy.20696","article-title":"Vascular anatomy of the developing medaka, Oryzias latipes: a complementary fish model for cardiovascular research on vertebrates","volume":"235","author":"Fujita M","year":"2006","journal-title":"Dev Dyn: Off Pub Am Assoc Anat"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-10-322446"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45851-3"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02416"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-03-274860"},{"key":"e_1_2_8_18_1","unstructured":"NCBI. 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