{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:14:51Z","timestamp":1791476091405,"version":"4.3.4"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:00:00Z","timestamp":1610668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Heart failure (HF) and cardiac hypertrophy is an unfavorable outcome of pathological cardiac remodeling and represents the most important contributing factor for HF and cardiac hypertrophy. Amygdalin (AMG) is a cyanogenic glycoside derived from bitter almonds. Accumulating evidences have highlighted their pharmacological potentials against various diseases. However, there is no report delineating the potential of AMG against angiotensin (Ang II) induced cardiac injuries. Thus, the present study was performed to explore whether AMG could ameliorate Ang II induced cardiomyopathies and thereby ascertain the underlying mechanisms thereof. To this end, H9c2 cells were treated with Ang II and thereafter treated with various concentration of AMG and finally the cardio\u2010protective effects of AMG were analyzed through Western blotting, immunofluorescence, and insilico analysis. Our results showed that the cardiomyocyte cell size, inflammatory markers and cytokines(pNF\u2010\u03baB, TNF\u2010\u03b1, iNOS and COX\u20102) were markedly increased following Ang II treatment; nevertheless, treatment with AMG led to considerable decrement in the Ang II induced enlargement of the cardiomyocytes, and attenuate the expression of hypertrophic markers(ANP, BNP and MHC\u20107), inflammatory markers and cytokines. Additionally, oxidative stress related proteins (Nrf2, catalase, SOD\u20102, and GPX\u20104) were markedly increased following AMG treatment. Molecular docking reveals the interaction of AMG with Nrf2 possessing good binding affinity. Cumulatively, our study highlights the cardio\u2010protective role of AMG against Ang II induced cardiomyopathies, including oxidative stress and inflammation effects. The intriguing in vitro results warrants the need of further animal studies to truly ascertain their potentialities.<\/jats:p>","DOI":"10.1002\/tox.23094","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T23:41:19Z","timestamp":1610754079000},"page":"926-934","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Cardioprotective potential of amygdalin against angiotensin <scp>II<\/scp> induced cardiac hypertrophy, oxidative stress and inflammatory responses through modulation of Nrf2 and <scp>NF\u2010\u03baB<\/scp> activation"],"prefix":"10.1002","volume":"36","author":[{"given":"Yen\u2010Lun","family":"Kung","sequence":"first","affiliation":[{"name":"Integration of Chinese medicine and Modern medicine Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"},{"name":"Department of Chinese Medicine Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010You","family":"Lu","sequence":"additional","affiliation":[{"name":"Cardiovascular and Mitochondrial Related Disease Research Center Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khan Fareen","family":"Badrealam","sequence":"additional","affiliation":[{"name":"Cardiovascular and Mitochondrial Related Disease Research Center Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2097-2240","authenticated-orcid":false,"given":"Wei\u2010Wen","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Biological Science and Technology College of Life Sciences, China Medical University  Taichung Taiwan"},{"name":"Ph.D. Program for Biotechnology Industry China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marthandam Asokan","family":"Shibu","sequence":"additional","affiliation":[{"name":"Cardiovascular and Mitochondrial Related Disease Research Center Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia Hsuan","family":"Day","sequence":"additional","affiliation":[{"name":"Department of Nursing MeiHo University  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray\u2010Jade","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Surgery School of Medicine, College of Medicine, Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang\u2010Yeh","family":"Lu","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, Department of Internal Medicine China Medical University Hospital  Taichung Taiwan"},{"name":"Graduate Institute of Biomedical Sciences, China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viswanadha Vijaya","family":"Padma","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Bharathiar University  Coimbatore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2347-0411","authenticated-orcid":false,"given":"Chih\u2010Yang","family":"Huang","sequence":"additional","affiliation":[{"name":"Cardiovascular and Mitochondrial Related Disease Research Center Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"},{"name":"Graduate Institute of Biomedical Sciences, China Medical University  Taichung Taiwan"},{"name":"Department of Biological Science and Technology Asia University  Taichung Taiwan"},{"name":"Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology  Hualien Taiwan"},{"name":"Department of Medical Research China Medical University Hospital, China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22589"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19061638"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201000080"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4588"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871520618666180105161136"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(80)90006-5"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2016.35"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.2197"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.14735\/amko2019360"},{"issue":"1","key":"e_1_2_6_11_1","first-page":"3","article-title":"Advanced research on anti\u2010tumor effects of amygdalin","volume":"10","author":"Song Z","year":"2014","journal-title":"J Cancer Res Ther"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.12.096"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.06.162"},{"issue":"10","key":"e_1_2_6_14_1","first-page":"1641","article-title":"Inhibitory effect of amygdalin on lipopolysaccharide\u2010inducible TNF\u2010alpha and IL\u20101beta mRNA expression and carrageenan\u2010induced rat arthritis","volume":"18","author":"Hwang HJ","year":"2008","journal-title":"J Microbiol Biotechnol"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12986-019-0356-5"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.4077\/CJP.2018.BAG544"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22421"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/1451676"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0897"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2020.02.016"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-017-4232-4"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvr159"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14129"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000254415.31362.a7"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000135475.35758.23"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044899"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/260429"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2009.11.001"}],"container-title":["Environmental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.23094","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/tox.23094","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.23094","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T17:16:59Z","timestamp":1693588619000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tox.23094"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,15]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1002\/tox.23094"],"URL":"https:\/\/doi.org\/10.1002\/tox.23094","archive":["Portico"],"relation":{},"ISSN":["1520-4081","1522-7278"],"issn-type":[{"value":"1520-4081","type":"print"},{"value":"1522-7278","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,15]]},"assertion":[{"value":"2020-09-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}