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Thyroid hormones controls neurodevelopment, which turn them sensitive to endocrine disruptors (EDs). In this work, we evaluated the effect of glyphosate\u2010based herbicides (GBH) as an intergenerational endocrine disrupter on thyroid homeostasis in cerebellar cells. Female pregnant Wistar rats were exposed to Roundup Transorb\u00ae solution at 5 and 50\u2009mg\/kg\/day, from gestation day 18 to post\u2010natal day 5 (P5). Cerebellum of male offspring was used to evaluate gene expression. The mRNA levels of thyroid hormone receptors, hormonal conversion enzymes, hormone transporters, as well as, de novo epigenetic regulators were altered, with some of these genes presenting a non\u2010monotonic dose response. Furthermore, metabolomic profile correlation with tested dose demonstrated altered metabolic profile, in agreement with cerebellar gene alterations. Moreover, cerebellar primary cultures exposed to non\u2010toxic GBH concentration presented a decrease level in glial fibrillary acidic protein, a protein regulated by endocrine signals. In conclusion, our results indicate that animals exposed to non\u2010toxic GBH doses during perinatal phase carry intergenerational alterations in key regulators of cellular thyroid hormone homeostasis and epigenetic controllers in adulthood, indicating the possible ED effect of GBH based on epigenetic alterations.<\/jats:p>","DOI":"10.1002\/tox.23102","type":"journal-article","created":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T11:05:20Z","timestamp":1611918320000},"page":"1031-1042","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Intergenerational thyroid hormone homeostasis imbalance in cerebellum of rats perinatally exposed to glyphosate\u2010based herbicide"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4555-3883","authenticated-orcid":false,"given":"Lu\u00e3 Tain\u00e3","family":"Costa Reis","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de 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